Antimicrobial activity and enzymatic analysis of endophytes isolated from Codonopsis pilosula
文献类型： 外文期刊
作者： Lodi, Rathna Silviya 1 ; Dong, Xiaodan 1 ; Jiang, Chunhui 1 ; Sun, Zhou 1 ; Deng, Peng 1 ; Sun, Sujun 1 ; Wang, Xinkun 1 ; Wang, Hengzhen 1 ; Mesa, Arundathi 4 ; Huang, Xiaopeng 2 ; Peng, Lizeng 1 ; Peng, Chune 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Food & Nutr Sci & Technol, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc Minist Agr, 23788 Gongye NRd, Jinan 250100, Shandong, Peoples R China
2.Gansu Agr Univ, Coll Mech & Elect Engn, 1, Yingmen Village, Lanzhou 730070, Gansu, Peoples R China
3.Qilu Univ Technol, Shandong Acad Sci, Acad Adv Interdisciplinary Studies, Daxue Rd, Jinan 250300, Shandong, Peoples R China
4.Yogi Vemana Univ, Dept Genet & Genom, Vemanapuram 516005, Andhra Pradesh, India
关键词： antimicrobial activity; Codonopsis pilosula; endophytes; enzymatic activity; secondary metabolites
期刊名称：FEMS MICROBIOLOGY ECOLOGY （ 影响因子：4.2； 五年影响因子：5.0 ）
ISSN： 0168-6496
年卷期： 2023 年 99 卷 8 期
页码：
收录情况： SCI
摘要： Endophytes of Codonopsis pilosula and their potentiality to produce antimicrobial secondary metabolites and enzymes. The roots of the medicinal plant Codonopsis pilosula (Franch.) Nannf (C. pilosula) possess most medicinal supplements. In current research on C. pilosula root endophytes were isolated, identified, and evaluated for their antimicrobial activity against human pathogens such as Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Pseudomonas aeruginosa and the fungi Candida albicans and Aspergillus niger. Endophytes C.P-8 and C.P-20 exhibited very significant antimicrobial activity, the secondary metabolite of C.P-8 registered at retention time 24.075 by HPLC analysis. Significant minimum inhibitory concentration (MIC) of C.P-8 was exhibited at 250 & mu;g/ml against S. aureus and 500 & mu;g/ml against B. subtilis. Qualitative, quantitative analyses, and partial purification of enzymes and purity was analysed by molecular weight determined by SDS-PAGE of enzymes produced by C.P-20, amylase-64 kDa, protease-64 kDa, chitinase-30 kDa, and cellulase-54 kDa. Optimum pH and temperature of the partially purified enzymes, was carried out. The partially purified enzymes from C.P-20 displayed maximum activity at pH 6-7 and temperatures of 40 & DEG;C-45 & DEG;C. Moreover, the above endophytes will be useful tools for producing active enzymes and active bioantimicrobial agents against human pathogens.
- 相关文献
作者其他论文 更多>>
-
Quinoline-derived electron-donating/withdrawing fluorophores for hydrazine detection and applications in environment and bioimaging
作者：Rajalakshmi, Kanagaraj;Muthusamy, Selvaraj;Xie, Meng;Xie, Jimin;Xu, Yuanguo;Kannan, Palanisamy;Lee, Ho-Jin;Zhu, Dongwei;Lodi, Rathna Silviya;Song, Jong-Won
关键词：Quinoline-2; 4-dintrobenzoate; Nucleophilic substitution; Intramolecular charge transfer; Soil samples; Isoniazid-hydrazine
-
Characterization of a polysaccharide from Polygala tenuifolia willd. with immune activity via activation MAPKs pathway
作者：Yu, Shasha;Ma, Rongjie;Ji, Haiyu;Yu, Juan;Liu, Anjun;Dong, Xiaodan;Liu, Anjun
关键词：Polygala tenuifolia Willd; Polysaccharide; Structural characteristic; Immunomodulatory activity
-
Trichoderma hamatum and Its Benefits
作者：Lodi, Rathna Silviya;Peng, Chune;Dong, Xiaodan;Deng, Peng;Peng, Lizeng
关键词：Trichoderma hamatum (Bonord.) Bainier; antimicrobial activity; antioxidant activity; insecticidal activity; herbicidal activity; plant growth promotion
-
Novel compounds with promising IDO1 inhibitory activity as new cancer drug candidates: Derivatives of N,N'-diphenylurea linked with 1,2,3-triazole
作者：Sun, Bin;Liu, Xiaofei;Sun, Xiuwei;Mao, Longfei;Shi, Weichen;Deng, Peng;Liu, Xiaofei;Sun, Xiuwei;Zhou, Yingjie;Mao, Longfei;Gong, Xiaoqing;Shi, Weichen;Shi, Lin;Shi, Lin
关键词：
-
A dual-mode nanoprobe based on silicon nanoparticles and Fe(II)-phenanthroline for the colorimetric and fluorescence determination of nitrite
作者：Yang, Chunlei;Xu, Guiju;Hou, Chenghao;Peng, Lizeng;Wang, Weiting;Zhang, Hongwei;Zhang, Xiaoling
关键词：Fluorescence silicon nanoparticles; Nitrite detection; Fluorometric method; Colorimetry; Nanoprobe
-
Preparation and structural characterization of acid-extracted polysaccharide from Grifola frondosa and antitumor activity on S180 tumor-bearing mice
作者：Zhang, Haibo;Yu, Juan;Liu, Anjun;Dong, Xiaodan;Ji, Haiyu;Yu, Juan;Liu, Anjun
关键词：Acid-extracted polysaccharide; G; frondosa; Antitumor activities
-
Development of a quinoline-derived turn-on fluorescent probe for real time detection of hydrazine and its applications in environment and bioimaging
作者：Muthusamy, Selvaraj;Rajalakshmi, Kanagaraj;Meng, Suci;Xie, Meng;Xie, Jimin;Xu, Yuanguo;Yin, Siqi;Zhu, Dongwei;Yin, Siqi;Zhu, Dongwei;Lodi, Rathna Silviya
关键词：Quinoline-2; 4-dinitrobenzoate; Hydrazine -toxic; ICT-AIE; Smartphone; Real sample; In situ hydrazine
