Insights into the Mechanism Underlying the Influence of Glycation with Different Saccharides and Temperatures on the IgG/IgE Binding Ability, Immunodetection, In Vitro Digestibility of Shrimp (Litopenaeus vannamei) Tropomyosin
文献类型: 外文期刊
作者: Zhao, Jinlong 1 ; Wang, Jin 2 ; Xu, Lili 4 ; Wang, Hao 1 ; Zhang, Ziye 1 ; Lin, Hong 1 ; Li, Zhenxing 1 ;
作者机构: 1.Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China
2.Southeast Univ, Sch Publ Hlth, Minist Educ, Key Lab Environm Med & Engn, Nanjing 210009, Peoples R China
3.Southeast Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Nanjing 210009, Peoples R China
4.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 Gongye North Rd, Jinan 250100, Peoples R China
关键词: shrimp (Litopenaeus vannamei); tropomyosin; glycation modification; IgG/IgE binding ability; immunodetection; in vitro digestibility; structure changes
期刊名称:FOODS ( 影响因子:5.2; 五年影响因子:5.5 )
ISSN:
年卷期: 2023 年 12 卷 16 期
页码:
收录情况: SCI
摘要: Tropomyosin (TM) is a heat-stable protein that plays a crucial role as a major pan-allergen in crustacean shellfish. Despite the high thermal stability of the TM structure, its IgG/IgE binding ability, immunodetection, and in vitro digestibility can be negatively influenced by glycation during food processing, and the underlying mechanism remains unclear. In this study, TM was subjected to glycosylation using various sugars and temperatures. The resulting effects on IgG/IgE-binding capacity, immunodetection, and in vitro digestibility were analyzed, meanwhile, the structural alterations and modifications using spectroscopic and LC-MS/MS analysis were determined. Obtained results suggested that the IgG/IgE binding capacity of glycosylated TM, immunodetection recovery, and in vitro digestibility were significantly reduced depending on the degree of glycosylation, with the greatest reduction occurring in Rib-TM. These changes may be attributable to structural alterations and modifications that occur during glycosylation processing, which could mask or shield antigenic epitopes of TM (E3: 61-81, E5b: 142-162, and E5c: 157-183), subsequently reducing the immunodetection recognition and digestive enzyme degradation. Overall, these findings shed light on the detrimental impact of glycation on TMs potential allergenicity and digestibility immunodetection and provide insights into the structural changes and modifications induced by thermal processing.
- 相关文献
作者其他论文 更多>>
-
Influence of linoleic acid on the immunodetection of shrimp (Litopenaeus vannamei) tropomyosin and the mechanism investigation via multi-spectroscopic and molecular modeling techniques
作者:Zhao, Jinlong;Wang, Hao;Zhang, Ziye;Lin, Hong;Li, Zhenxing;Zhao, Jinlong;Zhao, Jinlong;Liu, Yuhai;Xu, Lili;Sun, Lirui;Chen, Guanzhi;Li, Zhenxing
关键词:Shrimp(Litopenaeusvannamei) tropomyosin; Linoleic acid; Matrix effect; Immunodetection; Spectroscopic analysis; Molecular modeling
-
Comparison of tropomyosin released peptide and epitope mapping after in vitro digestion from fish (Larimichthys crocea), shrimp (Litopenaeus vannamei) and clam (Ruditapes philippinarum) through SWATH-MS based proteomics
作者:Xu, LiLi;Wen, Yun Qi;Zhao, Jin Long;Lin, Hong;Li, Zhen Xing;Zhang, Xiao Mei;Zhang, Hong Wei;Xu, LiLi;Xu, Tong Cheng;Yong, Ling
关键词:Tropomyosin; In vitro digestion; Cross-reactivity; SWATH-MS based proteomics; Peptide marker; Epitope
-
Research advances of SERS analysis method based on silent region molecules for food safety detection
作者:Sun, Yuhang;Zheng, Xinxin;Wang, Hao;Yang, Qinzheng;Sun, Yuhang;Wang, Hao;Yan, Mengmeng;Chen, Zilei;Shao, Yong
关键词:SERS; Silent region molecules; Matrix effects; Food safety
-
Multicolor aptasensors for pesticide multiresidues detection in agricultural products using bioorthogonal surface-enhanced Raman scattering tags
作者:Yan, Mengmeng;Wang, Hao;Li, Min;Zhang, Wenjun;Du, Hongxia;Chen, Zilei;Zhu, Chao;Yan, Mengmeng;Wang, Hao;Li, Min;Zhang, Wenjun;Du, Hongxia;Chen, Zilei;Zhu, Chao;She, Yongxin
关键词:Multiplex analysis; Matrix effect; Biologically Raman-silent region; 4-Ethenylbenzenamine; 2-Amino-4-cyanopyridine; Prussian blue
-
An interference-free SERS-based aptasensor for chlorpyrifos detection
作者:Wang, Hao;Chen, Zilei;Zhu, Chao;Du, Hongxia;Mao, Jiangsheng;Qin, Hongwei;Yan, Mengmeng;Wang, Hao;Chen, Zilei;Zhu, Chao;Du, Hongxia;Mao, Jiangsheng;Qin, Hongwei;Yan, Mengmeng;She, Yongxin
关键词:Interference -free; Aptasensor; Au@PB NPs; Anti -matrix effect; SERS tags
-
Capability of Phenology-Based Sentinel-2 Composites for Rubber Plantation Mapping in a Large Area with Complex Vegetation Landscapes
作者:Li, Hongzhong;Zhao, Longlong;Sun, Luyi;Li, Xiaoli;Han, Yu;Chen, Jinsong;Wang, Jin;Liang, Shouzhen
关键词:rubber plantations; Sentinel-2; feature importance; phenology; image compositing
-
An Alkyne-Mediated SERS Aptasensor for Anti-Interference Ochratoxin A Detection in Real Samples
作者:Wang, Hao;Chen, Lu;Li, Min;Zhu, Chao;Yan, Mengmeng;Wang, Hao;Chen, Lu;Li, Min;Zhu, Chao;Yan, Mengmeng;She, Yongxin
关键词:SERS; 4-TEAE; aptamer; silent region; OTA; anti-interference