您好，欢迎访问山东省农业科学院文献资源数据库平台

Two Microsatellite Types Within NAD6 Gene Help to Distinguish Populations and Infer the Migratory Route of the Invasive Fall Armyworm, Spodoptera frugiperda, (Lepidoptera, Noctuidae) in China

文献类型： 外文期刊

作者： Li, Chao 1 ; Song, Yingying 1 ; Li, Lili 1 ; Tessnow, Ashley E. 2 ; Zhu, Junsheng 4 ; Guan, Xiumin 4 ; Guo, Wenxiu 1 ; Cui, Hongying 1 ; Lu, Zengbin 3 ; Lv, Suhong 1 ; Yu, Yi 1 ; Men, Xingyuan 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Jinan, Shandong, Peoples R China

2.Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

3.Shandong Acad Agr Sci, Maize Res Inst, Jinan, Shandong, Peoples R China

4.Shandong Plant Protect Stn, Jinan, Shandong, Peoples R China

关键词： invasive pest; mitochondrial DNA; microsatellite; genotyping; geographic distribution

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.447； 五年影响因子：2.483 ）

ISSN： 0022-0493

年卷期：

页码：

收录情况： SCI

摘要： Spodoptera frugiperda is a major agricultural pest that has invaded China since January 2019. Given that most of the individuals present in China carried the diagnostic rice-strain mtDNA (COI-RS), there was no efficient method to distinguish populations of S. frugiperda. In this study, we identified and characterized two variant microsatellite alleles in the mitochondrial NAD6 gene of S. frugiperda retrieved from the National Center for Biotechnology Center GenBank. We then sequenced partial NAD6 genes containing the microsatellite region and the diagnostic COI barcoding gene (used to distinguish the corn-strain and the rice-strain) of 429 invasive S. frugiperda individuals that were collected from the main infested regions in China during 2019-2020. Our data indicates that two kinds of interrupted repeat sequences, (ATA)(4)T(ATA)(3) and (ATA)(5)T(ATA)(3), exist in the microsatellite region which we defined as the deletion type (NAD6-D), and the insertion type (NAD6-I) based on the repeat units' differentiation, respectively. The presence of these two microsatellite types in the mtDNA genome of S. frugiperda was further confirmed with the sequencing results in 429 samples. Moreover, NAD6-I and NAD6-D types were both present in individuals with COI-RS, while only NAD6-D type was detected in the COI-CS individuals. Interestingly, the two microsatellite types suggested a possible geographic distribution: the western migratory route (Yunan and Chongqing) was comprised exclusively of NAD6-I type, while both NAD6-I and NAD6-D types were identified in the predicted eastern migration trajectories (Hainan, Guangxi, Shandong, etc.). These results suggested that NAD6-D and NAD6-I types may be useful in distinguishing between populations, analyzing the evolutionary mechanism of mtDNA microsatellite polymorphism, inferring the migratory route of S. frugiperda in China, and developing precise and integrated control strategies for S. frugiperda.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台