Determination of extractable and non-extractable florfenicol residues as florfenicol amine in eggs by UPLC-MS/MS
文献类型： 外文期刊
作者： Li, Xia 1 ; Zhang, Yu 1 ; Sun, Qifan 1 ; Ding, Min 1 ; Li, Zengmei 1 ; Yue, Hui 1 ; Deng, Ligang 1 ; Chen, Lu 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, 202 Gongyebeilu Rd, Jinan 250100, Shandong, Peoples R China
2.Shandong Prov Key Lab Test Technol Food Qual & Sa, Jinan, Peoples R China
3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
关键词： Non-extractable florfenicol residues; florfenicol amine; acid-hydrolysis; ultrahigh-performance liquid chromatography-tandem mass spectrometry
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：3.549； 五年影响因子：3.542 ）
ISSN： 1944-0049
年卷期：
页码：
收录情况： SCI
摘要： Some florfenicol (FF) metabolites have a strong binding affinity towards biomolecules in the edible tissues of some food animals. These bound FF residues cannot be extracted directly from edible tissues with organic solvents and are present in higher concentrations even than solvent extractable residues. In this study, an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to detect the total residues of FF in eggs, by quantifying the metabolite florfenicol amine (FFA). The sample was hydrolyzed at 95-100 degrees C for 4 hours to release sample-matrix bound residues and convert them all into FFA. The hydrolyzed sample was washed with ethyl acetate to remove interfering substances, extracted with ethyl acetate under alkaline conditions, purified by solid phase extraction and quantified by UPLC-MS/MS. The recoveries of FFA in eggs ranged from 91.2 to 102.4%, with an RSD <= 10.9%. The LOD and LOQ were 0.5 and 1.0 mu g/kg, respectively. This method can be applied to the quantification of total FF residues in eggs.
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