Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay
文献类型： 外文期刊
作者： Sui, Xinxia 1 ; Wang, Linhai 1 ; Xia, Xianchun 1 ; Wang, Zhenlin 3 ; He, Zhonghu 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China
3.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
4.Chinese Acad Agr Sci, China Off, Int Maize & Wheat Improvement Ctr CIMMYT, Beijing 100081, Peoples R China
关键词： Functional marker;LMW-GS;multiplex PCR;Triticum aestivum L
期刊名称：CROP & PASTURE SCIENCE （ 影响因子：2.286； 五年影响因子：2.507 ）
ISSN： 1836-5795
年卷期： 2010 年 61 卷 12 期
页码：
收录情况： SCI
摘要： Low-molecular-weight glutenin subunits (LMW-GS) have significant effects on the processing quality of enduse products of common wheat. It is more efficient to discriminate LMW-GSalleles with PCR-based molecular markers than with SDS-PAGE. In the present study, we developed an allele-specific PCR marker, designated Glu-B3abefg, which can be used to discriminate protein alleles Glu-B3a, b,e,f, and g simultaneously. Based on ten previously developed allele-specific STS markers, three multiplex PCRs, viz. Glu-B3c + Glu-B3d, Glu-B3b + Glu-B3g, and Glu-B3h + Glu-B3i, were established. Six Glu-B3 alleles (b, c, d, g, h, and i) could be discriminated using the three multiplex PCRs. Results of tests on 158 wheat varieties and lines using Glu-B3abefg and the three multiplex PCRs were consistent with those using the ten STS markers separately. The new allele-specific marker and three multiplex PCRs represent an efficient way to undertake marker-assisted selection of Glu-B3 alleles.
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