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Chromosome arm-specific markers from Aegilops searsii permits targeted introgression

文献类型： 外文期刊

作者： Gong, Wenying 1 ; Gong, Wenping 1 ; Han, Ran 1 ; Li, Genying 1 ; Sehgal, Sunish Kumar 2 ; Li, Haosheng 1 ; Liu, Aifeng 1 ;

作者机构： 1.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China

2.S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA

关键词： Aegilops searsii;chromosome arm-specific molecular marker;genomic in situ hybridization;wheat breeding

期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）

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收录情况： SCI

摘要： Aegilops searsii (2n = 2x = 14, (SSs)-S-s), a wild relative of wheat, is a potentially important source of agriculturally useful genes for wheat breeding. The incorporation of Ae. searsii chromosome segments 1S(s) or 2S(s) into the wheat genome could significantly improve wheat flour quality and powdery mildew resistance, respectively. However, monitoring of wheatAe. searsii introgressions involving 1S(s) or 2S(s) chromosomes is difficult due to the lack of Ae. searsii-specific molecular markers. In this study, a total of 476 EST-or SSR-based primer pairs were synthesized and screened against a set of Chinese Spring (CS)-Ae. searsii addition lines and the control background variety CS. As a result, 17 molecular markers specific either for 1S(s) or 2S(s) chromosomes have been developed, among them, 1, 4, 2 and 10 markers have been assigned to chromosome arms 1S(s)S, 1S(s)L, 2S(s)S and 2S(s)L, respectively. All 17 markers can be successfully used to screen segregating populations. These markers are completely consistent with genomic in situ hybridization (GISH) results, indicating that these markers could be used for detecting Ae. searsii chromatin in wheat background.

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