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Exploring genotype-phenotype relationships of the LHX3 gene on growth traits in beef cattle

文献类型: 外文期刊

作者: Huang, Yong-Zhen 1 ; Jing, Yong-Jie 1 ; Sun, Yu-Jia 1 ; Lan, Xian-Yong 1 ; Zhang, Chun-Lei 2 ; Song, En-Liang 3 ; Chen 1 ;

作者机构: 1.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China

2.Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China

3.Shandong Acad Agr Sci, Inst Anim Husb & Vet, Jinan 250100, Shandong, Peoples R China

关键词: Association analyses;Cattle;Growth trait;Haplotype;LHX3

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The LIM-homeobox gene 3 (LHX3) plays an essential role in pituitary gland and nervous system development. Sequence variants (SVs) in coding and non-coding regions of LHX3 gene have an impact on LHX3 transcription and growth traits in cattle. Previously, we have identified 3 single nucleotide polymorphisms (SNPs: 1-3) in all exons and intron 2 regions of the LHX3 gene in cattle. Here, 7 novel SNPs (SNPs: 4-10) were identified by DNA sequencing and polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) methods. In the present study, a total of 10 SNPs were assessed linkage disequilibrium (LD) in 802 cows representing four main cattle breeds from China (Nanyang, Qinchuan, Jiaxian, and Chinese Holstein). The assessment results demonstrated that 17 haplotypes and 18 diplotypes were revealed in these cattle populations. Moreover, association analysis indicated that the genotypes of SNPs 1-6 are associated with the body weight at 6, 12 and 18. months of age in Nanyang cattle (P < 0.01 or P < 0.05), whereas no significant association was found between the 18 diplotypes and growth traits. Our results provide evidence that some SNPs in LHX3 gene may be associated with body weight at certain age, and LHX3 gene may be used as candidate gene for marker-assisted selection (MAS) in beef cattle breeding.

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