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Size-dependent modulation of graphene oxide-aptamer interactions for an amplified fluorescence-based detection of aflatoxin B-1 with a tunable dynamic range

文献类型： 外文期刊

作者： Zhang, JingJing 1 ; Li, Zengmei 2 ; Zhao, Shancang 2 ; Lu, Yi 1 ;

作者机构： 1.Univ Illinois, Dept Chem, Urbana, IL 61801 USA

2.Shandong Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Jinan 250100, Peoples R China

3.Key Lab Test Technol Food Qual & Safety Shandong, Dept Chem, Jinan 250100, Peoples R China

期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）

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收录情况： SCI

摘要： Aflatoxin B-1 (AFB(1)) is a common toxin found in many foods. While AFB(1) sensors have been reported, few studies have shown amplified detection with tunable dynamic ranges. We herein report a simple and highly sensitive amplified aptamer-based fluorescent sensor for AFB(1), which relies on the ability of nano-graphene oxide (GO) to protect aptamers from nuclease cleavage for amplified detection and on the nanometer size effect of GO to tune the dynamic range and sensitivity. The assay was performed by simply mixing the carboxyl-X-rhodamine (ROX)-labeled AFB(1) aptamer, the GO, the nuclease, and the AFB1 samples. Modulating the size of the GO nanosheet resulted in three dynamic ranges, i.e., 12.5 to 312.5 ng mL(-1), 1.0 to 100 ng mL(-1), and 5.0 to 50 ng mL(-1), with corresponding limits of detection of 10.0 ng mL(-1), 0.35 ng mL(-1) and 15.0 ng mL(-1), respectively. The sensor was highly selective against other aflatoxins and common molecules in foods, and its performance was verified in corn samples spiked with known concentration of AFB(1).

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