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Purification and characterization of a ribonuclease with antiproliferative activity from the mystical wild mushroom Tuber indicum

文献类型： 外文期刊

作者： Xiao, Chen 2 ; Feng, ShanShan 1 ; Wang, HeXiang 1 ; Gong, ZhiYuan 3 ; Ng, TziBun 4 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

3.Shandong Acad Agr Sci, Inst Agr Resources & Environm, Jinan, Shandong, Peoples R China

4.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China

关键词： Antiproliferative;Purification;Ribonuclease;Truffle;Tuber indicum

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： An RNase with a molecular mass of 28kDa and with high ribonucleolytic activity toward poly(A) was purified from the ascocarps of Tuber indicum. The purification procedure involved ion exchange chromatography on diethylaminoethyl cellulose, Q-Sepharose and Mono Q, and gel filtration by fast protein liquid chromatography on Superdex 75. The pH and temperature optima of the RNase were 7.2 and 50°C, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly(A)>poly(C)>poly(G)≈poly(U). All of the metal ions used in this study, except for the K+ ions, curtailed the activity of the RNase. The RNase activity was reduced by ethylene diamine tetraacetic acid (EDTA), dithiothreitol (DTT), and sodium dodecyl sulfate (SDS) by 42.2%, 75.5%, and 96.6%, respectively. The RNase inhibited the proliferation of hepatoma (HepG2) and human breast cancer cell lines (MCF7), with half-maximal inhibitory concentrations (IC50) of 12.6 and 16.6μM, respectively.

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