您好，欢迎访问山东省农业科学院文献资源数据库平台

A convenient sandwich assay of thrombin in biological media using nanoparticle-enhanced fluorescence polarization

文献类型： 外文期刊

作者： Yue, Qiaoli 1 ; Shen, Tongfei 1 ; Wang, Lei 1 ; Xu, Shuling 1 ; Li, Haibo 1 ; Xue, Qingwang 1 ; Zhang, Yuanfu 1 ; Gu, Xia 1 ;

作者机构： 1.Liaocheng Univ, Dept Chem, Liaocheng 252059, Peoples R China

2.Shandong Acad Agr Sci, Shandong Prov Key Lab Test Technol Food Qual & Sa, Jinan 250100, Peoples R China

关键词： Silica nanoparticle;Fluorescence polarization;Plasma;Aptamer;Thrombin

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A new aptamer biosensor was presented for the detection of thrombin in this work, which was based on fluorescence polarization (FP) using silica nanoparticles as enhancement probe. The silica nanoparticles covered by streptavidin were tagged with a thrombin aptamer (5'-biotin-GGTTGGTGTGGTTGG-3'), which was bound to the surface of silica nanoparticle through the specific interaction between streptavidin and biotin. In the presence of thrombin, it induced the aptamer to form quadruplex structure. When the other thrombin aptamer labeled with fluorescein (5'-FAM-AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3') was added to the above system, a sandwich structure can form at the surface of silica nanoparticles. The fluorescence polarization was therefore enhanced and quantification between fluorescence polarization signal and concentration of thrombin was built. The sensor provided a linear range from 0.6 to 100 nM for thrombin with a detection limit of 0.20 nM (3.29S_B/m,according to the recent recommendation of IUPAC) in a homogeneous media. The same linear range was obtained in spiked human serum samples with a slightly higher detection limit (0.26 nM), demonstrating high anti-interference of the sensor in a complex biological sample matrix. And the sensor can be used to monitor spiked concentration of thrombin level in real human plasma with satisfactory results obtained.

  • 相关文献

[1]Zinc Oxide Nanoparticle Caused Plasma Metabolomic Perturbations Correlate with Hepatic Steatosis. Zhang, Weidong,Zhao, Yong,Li, Lan,Feng, Yanni,Min, Lingjiang,Ma, Dongxue,Yu, Shuai,Shen, Wei,Zhao, Yong,Zhang, Hongfu,Li, Fuli,Liu, Jing,Shi, Tianhong,Li, Fuwei. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台