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A reusable and sensitive biosensor for total mercury in canned fish based on fluorescence polarization

文献类型： 外文期刊

作者： Shen, Tongfei 1 ; Yue, Qiaoli 1 ; Jiang, Xiuxiu 1 ; Wang, Lei 1 ; Xu, Shuling 1 ; Li, Haibo 1 ; Gu, Xiaohong 2 ; Zhang, S 1 ;

作者机构： 1.Liaocheng Univ, Dept Chem, Liaocheng 252059, Peoples R China

2.Shandong Acad Agr Sci, Shandong Prov Key Lab Test Technol Food Qual & Sa, Jinan 250100, Peoples R China

关键词： Canned fish;Fluorescence;Magnetic nanoparticles;Mercury;Polarization

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： In this work, we developed a sensitive and selective sensor technique for total mercury (Hg) detection in canned fish samples based on the fluorescence polarization (FP) method. The detection principle was that ssDNA containing thymine (T) bases was modified on magnetic nanoparticles (MNPs), which were used as enhancement probe. In the presence of Hg~(2+), the ssDNA on MNPs can hybridize with the fluorophore labeled aptamer owing to the specific interaction between T bases and Hg~(2+). The formation of thymine-Hg~(2+)-thymine (T-Hg~(2+)-T) complexes leads to the molar mass increase of fluorophore molecules, resulting in the enhancement of FP signal. The increase of FP was in a good linearity with the concentration of Hg~(2+) in range of 2.0 nM~(-1).0 mM and the limit of detection was 0.49 nM (3.29 S_B/m, according to the recent recommendation of IUPAC). Moreover, the proposed biosensor can be reused for 6 cycling times and was successfully applied in monitoring Hg~(2+) in real samples.

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