Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation
文献类型： 外文期刊
作者： Tan, Xiuwen 1 ; Song, Enliang 1 ; Liu, Xiaomu 1 ; Liu, Guifen 1 ; Cheng, Haijian 1 ; Wan, Fachun 1 ;
作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Inst Anim Husb & Vet Med, Jinan 250100, Shandong, Peoples R China
关键词： Vitrification;Concentration;Supercool X-1000;Ovaries
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）
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收录情况： SCI
摘要： The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5%ethylene glycol (EG)+12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG+10% DMSO or 15% EG+15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.
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