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Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay

文献类型： 外文期刊

作者： Lu, Xiao 2 ; Zheng, Hong 1 ; Li, Xiu-Qing 2 ; Yuan, Xue-Xia 2 ; Li, Hui; Deng, Li-Gang 2 ; Zhang, Hong 2 ; Wang, Wen-Z 1 ;

作者机构： 1.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

2.Shandong Acad Agr Sci, Cent Lab, Jinan 250100, Peoples R China

3.Key Lab Test Technol Food Qual & Safety Shandong, Jinan 250100, Peoples R China

4.Key Lab Test Techn

关键词： ractopamine;pork;matrix effect;surface plasmon resonance;biosensor;UPLC-MS/MS

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

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收录情况： SCI

摘要： A surface plasmon resonance (SPR) biosensor inhibition immunoassay for determination of ractopamine (Rac) residue in pork was constructed by immobilising Rac derivative on the SPR-2004 biosensor chip. After extraction with perchloric acid, pork sample was cleaned by ethyl acetate and analyzed by SPR-2004 biosensor. The limit of detection (LOD) was 0.6 ug/kg for pork sample. Recoveries of Rac were higher than 80% with relative standard deviations below 10%. Although the same pretreatment was applied for both the UPLC-MS/MS and the biosensor, the biosensor showed little matrix interference by constructing pure solution and matrix-match calibration curves. Compared with Biacore Q made by Biacore AB, SPR-2004 biosensor made by Chinese Academy of Sciences which is of low cost and showed minimal matrix interferences. Accordingly, this biosensor was a promising screening instrument to be used for detection of ractopamine in supermarkets, food factories and food regulatory organisations.

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