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Molecular cloning, promoter analysis, SNP detection of Clusterin gene and their associations with mastitis in Chinese Holstein cows

文献类型： 外文期刊

作者： Wang, Zeying 1 ; Huang, Jingmin 2 ; Zhong, Jifeng 2 ; Wang, Genlin 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

2.Shandong Acad Agr Sci, Ctr Dairy Cattle Sci Res, Jinan 250100, Peoples R China

关键词： Clusterin;Promoter;SNP;Haplotype;Milk production traits;Mastitis

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

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收录情况： SCI

摘要： To examine the effect of Clusterin (CLU) on mastitis, genetic association analysis was applied on mastitis and milk production traits of 1,137 Chinese Holstein cows. We detected two novel single nucleotide polymorphisms (SNPs), G+15781A in the seventh exon and C-994T before 5'-upstream region (UTR) of CLU gene, found five TATA box, one CpG island and more transcription factor binding sites in promoter region, respectively, Milk fat rate in genotype AA was significantly higher than in GG on fat rate (P < 0.01), milk fat and milk yield in combined haplotype H1H4 (AGCT) were significantly higher than in H3H4 (GGCT) (P < 0.05), H1H4 was decided advantage in nine milk production traits. Quadruplet cows in G+15781A were decided advantage in each milk production traits that 305-day milk yield, fat rate, protein rate and somatic cell scores (SCS) increased following with birth order. A allele and T allele had positive effect on SCS. In conclusion, this study showed that the haplotype AA may be a genetic marker on mastitis and other performance for Chinese Holstein cows. Bovine mastitis is one of the most deleterious diseases inflammation of the mammary gland of dairy cattle and is usually a consequence of microbial infection and affected by genetic contagious [1]. It is a highly prevalent and costly disease for the dairy industry worldwide [2, 3]. Clusterin (CLU) was first described in 1983 as a major secretory glycoprotein produced by ram Sertoli cells [4]. It is normally secreted from mammalian cells and is both widely distributed and highly conserved. Between different mammals, about 70-80% of the amino acid sequence of Clusterin is identical [5]. One of the most striking things about CLU is the breadth of its biological distribution [6, 7]. In animal tissues CLU mRNA is near ubiquitous, including breast milk [8]. Furthermore, increased CLU expression is found in a variety of disease states [9], such as during mastitis of the dairy cow [10]. These implied that there was a possible relationship between the CLU and mastitis in dairy cows. So, it's of importance to examine the effect of CLU on mastitis for accelerating the breeding in Chinese Holstein cows.

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