您好，欢迎访问山东省农业科学院文献资源数据库平台

Preparation of anti-Sudan red monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for detection of Sudan red in chilli jam and chilli oil

文献类型： 外文期刊

作者： Xu, Jing 2 ; Zhang, Yuanyang 2 ; Yi, Jian 2 ; Meng, Meng 1 ; Wan, Yuping 3 ; Feng, Caiwei 3 ; Wang, Shanliang 3 ; Lu, Xia 1 ;

作者机构： 1.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China

2.Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Shandong, Peoples R China

3.Beijing Wanger Biotechnol Co LTD, Beijing 102206, Peoples R China

4.Shandong Acad Agr Sci, Cent Lab, Jinan 250100, Shandong, Peoples R China

期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Sudan dyes are banned to be used in food additives because of the carcinogenicity of their metabolites. A rapid and sensitive indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed to detect the residues of Sudan dyes. Novel immunogen and coating antigen were synthesized via glutaraldehyde linking. The hapten-bovine serum albumin (BSA) was applied as immunogen and the hapten-ovalbumin (OVA) was served as coating antigen. The monoclonal antibody obtained showed high sensitivity to Sudan I with an IC _(50) value of 1.7 μg L~(-1) in buffer and was suitable to detect the residues of Sudan red in food products. The specificity of the assay was studied by measuring cross-reactivity of the antibody with the structurally related compounds of Sudan II (<1%), Sudan IV (<1%) and para red (120%). Chilli jam and chilli oil samples spiked with Sudan dyes were analyzed by the method. The detection limit (LOD) of the ELISA method applied in chilli jam and chilli oil was 9.0 μg L~(-1) and 19.6 μg L~(-1), respectively. The recovery rates of Sudan-I in chilli oil and chilli jam were in the range of 80%-110% with coefficients of variation <25%. The intra-assay variation and inter-assay variation in buffer were both <9%.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台