您好，欢迎访问山东省农业科学院文献资源数据库平台

Gene Profiling in Late Blight Resistance in Potato Genotype SD20

文献类型： 外文期刊

作者： Yang, Xiaohui 1 ; Guo, Xiao 1 ; Yang, Yu 1 ; Ye, Pei 3 ; Xiong, Xingyao 2 ; Liu, Jun; Dong, Daofeng 1 ; Li, Guangcun 2 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Mol Biol Key Lab Shandong Facil Vegetable, Jinan 250100, Shandong, Peoples R China

2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Biol & Genet Improvement Tuber & Root Cro, Minist Agr, Beijing 100081, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil C

关键词： potato; late blight; Phytophthora infestans; transcriptome; resistance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2018 年 19 卷 6 期

页码：

收录情况： SCI

摘要： Late blight caused by the oomycete fungus Phytophthora infestans (Pi) is the most serious obstacle to potato (Solanum tuberosum) production in the world. A super race isolate, CN152, which was identified from Sichuan Province, China, could overcome nearly all known late blight resistance genes and caused serious damage in China. The potato genotype SD20 was verified to be highly resistant to CN152; however, the molecular regulation network underlying late blight resistance pathway remains unclear in SD20. Here, we performed a time-course experiment to systematically profile the late blight resistance response genes using RNA-sequencing in SD20. We identified 3354 differentially expressed genes (DEGs), which mainly encoded transcription factors and protein kinases, and also included four NBS-LRR genes. The late blight responsive genes showed time-point-specific induction/repression. Multi-signaling pathways of salicylic acid, jasmonic acid, and ethylene signaling pathways involved in resistance and defense against Pi in SD20. Gene Ontology and KEGG analyses indicated that the DEGs were significantly enriched in metabolic process, protein serine/threonine kinase activity, and biosynthesis of secondary metabolites. Forty-three DEGs were involved in immune response, of which 19 were enriched in hypersensitive response reaction, which could play an important role in broad-spectrum resistance to Pi infection. Experimental verification confirmed the induced expression of the responsive genes in the late blight resistance signaling pathway, such as WRKY, ERF, MAPK, and NBS-LRR family genes. Our results provided valuable information for understanding late blight resistance mechanism of potato.

  • 相关文献

[1]Gene Profiling in Late Blight Resistance in Potato Genotype SD20. Yang, Xiaohui,Guo, Xiao,Yang, Yu,Dong, Daofeng,Yang, Xiaohui,Xiong, Xingyao,Li, Guangcun,Ye, Pei,Liu, Jun. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台