Chromosome-scale genome assembly-assisted identification of Mi-9 gene in Solanum arcanum accession LA2157, conferring heat-stable resistance to Meloidogyne incognita
文献类型： 外文期刊
作者： Jiang, Lijun 1 ; Ling, Jian 1 ; Zhao, Jianlong 1 ; Yang, Yu 2 ; Yang, Yuhong 1 ; Li, Yan 1 ; Jiao, Yang 1 ; Mao, Zhenchuan 1 ; Wang, Yunsheng 4 ; Xie, Bingyan 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing, Peoples R China
2.Shandong Acad Agr Sci, Inst Vegetables, Jinan, Shandong, Peoples R China
3.Shandong Acad Agr Sci, Inst Modern Agr Yellow River Delta, Jinan, Shandong, Peoples R China
4.Hunan Agr Univ, Coll Plant Protect, Changsha, Hunan, Peoples R China
关键词： Solanum arcanum LA2157; Mi-9; genome assembly; Hi-C; VIGS; tomato genetic transformation
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）
ISSN： 1467-7644
年卷期： 2023 年 21 卷 7 期
页码：
收录情况： SCI
摘要： Root-knot nematodes (RKNs) are infamous plant pathogens in tomato production, causing considerable losses in agriculture worldwide. Mi-1 is the only commercially available RKN-resistance gene; however, the resistance is inactivated when the soil temperature is over 28 ?. Mi-9 in wild tomato (Solanum arcanum LA2157) has stable resistance to RKNs under high temperature but has not been cloned and applied. In this study, a chromosome-scale genome assembly of S. arcanum LA2157 was constructed through Nanopore and Hi-C sequencing. Based on molecular markers of Mi-9 and comparative genomic analysis, the localization region and candidate Mi-9 genes cluster consisting of seven nucleotide-binding sites and leucine-rich repeat (NBS-LRR) genes were located. Transcriptional expression profiles confirmed that five of the seven candidate genes were expressed in root tissue. Moreover, virus-induced gene silencing of the Sarc_034200 gene resulted in increased susceptibility of S. arcanum LA2157 to Meloidogyne incognita, and genetic transformation of the Sarc_034200 gene in susceptible Solanum pimpinellifolium conferred significant resistance to M. incognita at 25 ? and 30 ? and showed hypersensitive responses at nematode infection sites. This suggested that Sarc_034200 is the Mi-9 gene. In summary, we cloned, confirmed and applied the heat-stable RKN-resistance gene Mi-9, which is of great significance to tomato breeding for nematode resistance.
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