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Molecular Evidence of RNA Editing in Bombyx Chemosensory Protein Family

文献类型： 外文期刊

作者： Xuan, Ning 1 ; Bu, Xun 1 ; Liu, Yan Yan 1 ; Yang, Xue 1 ; Liu, Guo Xia 1 ; Fan, Zhong Xue 1 ; Bi, Yu Ping 1 ; Yang, Lian Qun 1 ; Lou, Qi Nian 2 ; Rajashekar, Balaji 3 ; Leppik, Getter 4 ; Kasvandik, Sergo 4 ; Picimbon, Jean-Francois 1 ;

作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Shandong Silkworm Inst, Yantai, Shandong, Peoples R China

3.Univ Tartu, Inst Comp Sci, EE-50090 Tartu, Tartumaa Provin, Estonia

4.Univ Tartu, Inst Technol, Prote Core Facil, EE-50090 Tartu, Tartumaa Provin, Estonia

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Chemosensory proteins (CSPs) are small scavenger proteins that are mainly known as transporters of pheromone/odor molecules at the periphery of sensory neurons in the insect antennae and in the producing cells from the moth female pheromone gland. Sequencing cDNAs of RNA encoding CSPs in the antennae, legs, head, pheromone gland and wings from five single individual adult females of the silkworm moth Bombyx mori showed that they differed from genomic sequences by subtle nucleotide replacement (RDD). Both intronless and intronic CSP genes expressed RDDs, although in different rates. Most interestingly, in our study the degree of RDDs in CSP genes were found to be tissue-specific. The proportion of CSP-RDDs was found to be significantly much higher in the pheromone gland. In addition, Western blot analysis of proteins in different tissues showed existence of multiple CSP protein variant chains particularly found in the pheromone gland. Peptide sequencing demonstrated the occurrence of a pleiad of protein variants for most of all BmorCSPs from the pheromone gland. Our findings show that RNA editing is an important feature in the expression of CSPs and that a high variety of RDDs is found to expand drastically thus altering the repertoire of CSP proteins in a tissue-specific manner.

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