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Aptamer-functionalized pH-responsive polymer-modified magnetic nanoparticles for specific enrichment and sensitive determination of lactoferrin

文献类型： 外文期刊

作者： Ma, Yao 1 ; Zhao, Liping 1 ; Li, Linsen 1 ; Zhu, Chao 2 ; Qu, Feng 1 ;

作者机构： 1.Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, 5 South Zhongguancun St, Beijing 100081, Peoples R China

2.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China

关键词： Lactoferrin; Aptamer; pH-responsive polymer; Magnetic nanoparticles; Preconcentration; HPLC

期刊名称：MICROCHIMICA ACTA （ 影响因子：6.408； 五年影响因子：5.888 ）

ISSN： 0026-3672

年卷期： 2023 年 190 卷 1 期

页码：

收录情况： SCI

摘要： A new type of aptamer-functionalized pH-responsive polymer-modified magnetic nanoparticles (ApMNPs) is introduced for specific enrichment and sensitive determination of lactoferrin (Lf) in complex matrixes. In the construction, Fe3O4@3-(Triethoxysilyl)propylmethacrylate@poly(4-Vinyl-1, 3-dioxolan-2-one-acrylic acid) (Fe3O4@MPS@p(VEC-AA)) were synthesized as pH-responsive polymer-modified magnetic nanoparticles (pMNPs) through free radical polymerization to increase the tunable interaction. Lf-binding aptamers were conjugated onto pMNPs through the reaction of amino-group in aptamer and epoxide-group in VEC, innovatively applied to prepare Lf-ApMNPs. On the basis of the synergistic effect of specific affinity of aptamer on Lf and tunable hydrophobic/hydrophilic property of pH-responsive polymer, Lf-ApMNPs presented good selectivity toward Lf, excellent adsorption capacity (as high as 233.9 mg g(-1)), as well as good recoveries in the range 93.6-99.6% in Lf-related nutrition samples. Significantly, the introduction of pH-responsive monomer (AA) effectively regulated the adsorption-desorption process of Lf, with the function similar to a switch. Moreover, the good performances of Ct-ApMNPs toward alpha-Chymotrypsin showed that ApMNPs exhibited universality to other proteins through easily changing the binding aptamer, thereby offering a facile and efficient approach for specific enrichment and sensitive determination of targets in real biological samples.

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