Aptamer-functionalized pH-responsive polymer-modified magnetic nanoparticles for specific enrichment and sensitive determination of lactoferrin
文献类型: 外文期刊
作者: Ma, Yao 1 ; Zhao, Liping 1 ; Li, Linsen 1 ; Zhu, Chao 2 ; Qu, Feng 1 ;
作者机构: 1.Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, 5 South Zhongguancun St, Beijing 100081, Peoples R China
2.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China
关键词: Lactoferrin; Aptamer; pH-responsive polymer; Magnetic nanoparticles; Preconcentration; HPLC
期刊名称:MICROCHIMICA ACTA ( 影响因子:6.408; 五年影响因子:5.888 )
ISSN: 0026-3672
年卷期: 2023 年 190 卷 1 期
页码:
收录情况: SCI
摘要: A new type of aptamer-functionalized pH-responsive polymer-modified magnetic nanoparticles (ApMNPs) is introduced for specific enrichment and sensitive determination of lactoferrin (Lf) in complex matrixes. In the construction, Fe3O4@3-(Triethoxysilyl)propylmethacrylate@poly(4-Vinyl-1, 3-dioxolan-2-one-acrylic acid) (Fe3O4@MPS@p(VEC-AA)) were synthesized as pH-responsive polymer-modified magnetic nanoparticles (pMNPs) through free radical polymerization to increase the tunable interaction. Lf-binding aptamers were conjugated onto pMNPs through the reaction of amino-group in aptamer and epoxide-group in VEC, innovatively applied to prepare Lf-ApMNPs. On the basis of the synergistic effect of specific affinity of aptamer on Lf and tunable hydrophobic/hydrophilic property of pH-responsive polymer, Lf-ApMNPs presented good selectivity toward Lf, excellent adsorption capacity (as high as 233.9 mg g(-1)), as well as good recoveries in the range 93.6-99.6% in Lf-related nutrition samples. Significantly, the introduction of pH-responsive monomer (AA) effectively regulated the adsorption-desorption process of Lf, with the function similar to a switch. Moreover, the good performances of Ct-ApMNPs toward alpha-Chymotrypsin showed that ApMNPs exhibited universality to other proteins through easily changing the binding aptamer, thereby offering a facile and efficient approach for specific enrichment and sensitive determination of targets in real biological samples.
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