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Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL

文献类型： 外文期刊

作者： Song, Jie 1 ; Xu, Dengan 2 ; Dong, Yan 1 ; Li, Faji 3 ; Bian, Yingjie 1 ; Li, Lingli 1 ; Luo, Xumei 1 ; Fei, Shuaipeng 1 ; Li, Lei 1 ; Zhao, Cong 1 ; Zhang, Yong 1 ; Xia, Xianchun 1 ; Ni, Zhongfu 4 ; He, Zhonghu 1 ; Cao, Shuanghe 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Natl Wheat Improvement Ctr, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China

2.Qingdao Agr Univ, Coll Agron, Shandong Prov Key Lab Dryland Farming Technol, Qingdao 266109, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Crop Res Inst, 202 Gongye North Rd, Jinan 250100, Shandong, Peoples R China

4.China Agr Univ, Beijing Key Lab Crop Genet Improvement, State Key Lab Agrobiotechnol, Key Lab Crop Heterosis & Utilizat,Coll Agron, 2 Yuanmingyuan West Rd, Beijing 100094, Peoples R China

5.CAAS, Int Maize & Wheat Improvement Ctr CIMMYT China Of, 12 Zhongguancun South St, Beijing 100081, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期：

页码：

收录情况： SCI

摘要： Key message We fine mapped QTL QTKW.caas-5DL for thousand kernel weight in wheat, predicted candidate genes and developed a breeding-applicable marker. Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection.

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