文献类型： 外文期刊

作者： Yuan, Xiao-Yuan ¹ ; Wang, You-Ling ¹ ; Meng, Kai ¹ ; Zhang, Yu-Xia ¹ ; Xu, Huai-Ying ¹ ; Ai, Wu ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Poultry Inst, Jinan, Shandong, Peoples R China; Poultry Breeding Engn Technol Ctr Shandong Prov, Shandong Prov Key Lab Poultry Dis Diag & Immunolo, Jinan, Shandong, Peoples R China

关键词： Adenovirus; LAMP; Real-time turbidity; Visual detection

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2019 年 15 卷

页码：

收录情况： SCI

摘要： BackgroundFowl adenovirus (FAdV) is an infectious agent that can cause jaundice, severe anaemia, dyspnoea and reproductive disorders in fowls. Since 2015, FAdV disease has been rapidly spreading among broiler chickens in China, where it has caused significant economic losses. In this study, a loop-mediated isothermal amplification (LAMP) real-time turbidity method with strong specificity to FAdV was established.ResultsThe established assay was specific to FAdV-4, and the lower limit of detection was 75 copies/mu L of extracted DNA. The positive detection rate for the suspected tissue samples was 33.3% (14/42), which was consistent with that of the real-time PCR.ConclusionThe proposed LAMP method can quickly and accurately detect prevalent FAdV via real-time turbidity assay, thereby providing a diagnostic platform for the prevention and control of the FAdV disease.