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Constitutive Expression of an Apple FLC3-like Gene Promotes Flowering in Transgenic Blueberry under Nonchilling Conditions

文献类型： 外文期刊

作者： Zong, Xiaojuan 1 ; Zhang, Yugang 1 ; Walworth, Aaron 1 ; Tomaszewski, Elise M. 1 ; Callow, Pete 1 ; Zhong, Gan-yuan 4 ;

作者机构： 1.Michigan State Univ, Dept Hort, Plant Biotechnol Resource & Outreach Ctr, E Lansing, MI 48824 USA

2.Shandong Acad Agr Sci, Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China

3.Qingdao Agr Univ, Coll Hort, Qingdao Key Lab Genet Improvement & Breeding Hort, Qingdao 266109, Shandong, Peoples R China

4.USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA

关键词： chilling requirement; dormancy release; FLOWERING LOCUS C; flowering time; MADS-box gene; woody plants

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2019 年 20 卷 11 期

页码：

收录情况： SCI

摘要： MADS-box transcription factors FLOWERING LOCUS C (FLC) and APETALA1 (AP1)/CAULIFLOWER (CAL) have an opposite effect in vernalization-regulated flowering in Arabidopsis. In woody plants, a functional FLC-like gene has not been verified through reverse genetics. To reveal chilling-regulated flowering mechanisms in woody fruit crops, we conducted phylogenetic analysis of the annotated FLC-like proteins of apple and found that these proteins are grouped more closely to Arabidopsis AP1 than the FLC group. An FLC3-like MADS-box gene from columnar apple trees (Malus domestica) (MdFLC3-like) was cloned for functional analysis through a constitutive transgenic expression. The MdFLC3-like shows 88% identity to pear's FLC-like genes and 82% identity to blueberry's CAL1 gene (VcCAL1). When constitutively expressed in a highbush blueberry (Vaccinium corymbosum L.) cultivar Legacy', the MdFLC3-like induced expressions of orthologues of three MADS-box genes, including APETALA1, SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1, and CAL1. As a consequence, in contrast to the anticipated late flowering associated with an overexpressed FLC-like, the MdFLC3-like promoted flowering of transgenic blueberry plants under nonchilling conditions where nontransgenic Legacy' plants could not flower. Thus, the constitutively expressed MdFLC3-like in transgenic blueberries functioned likely as a blueberry's VcCAL1. The results are anticipated to facilitate future studies for revealing chilling-mediated flowering mechanisms in woody plants.

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