Quantitative Proteomic and Transcriptomic Analyses of Metabolic Regulation of Adult Reproductive Diapause in Drosophila suzukii (Diptera: Drosophilidae) Females
文献类型： 外文期刊
作者： Zhai, Yifan 1 ; Dong, Xiaolin 2 ; Gao, Huanhuan 4 ; Chen, Hao 1 ; Yang, Puyun 5 ; Li, Ping 5 ; Yin, Zhenjuan 1 ; Zheng, L 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Jinan, Shandong, Peoples R China
2.Yangtz Univ, Coll Agr, Jingzhou, Peoples R China
3.Shandong Normal Univ, Coll Life Sci, Jinan, Shandong, Peoples R China
4.Shandong Acad Grp, Jinan, Shandong, Peoples R China
5.Natl Agrotech Extens & Serv Ctr, Beijing, Peoples R China
关键词： Drosophila suzukii; quantitative proteomics; RNA-Seq transcriptomics; reproductive diapause; qRT-PCR
期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）
ISSN： 1664-042X
年卷期： 2019 年 10 卷
页码：
收录情况： SCI
摘要： Diapause is a form of dormancy used by many insects to survive adverse environmental conditions, which can occur in specific developmental stages in different species. Drosophila suzukii is a serious economic pest and we determined the conditions for adult reproductive diapause by the females in our previous studies. In this study, we combined RNA-Seq transcriptomic and quantitative proteomic analyses to identify adult reproductive diapause-related genes and proteins. According to the transcriptomic analysis, among 242 annotated differentially expressed genes in non-diapause and diapause females, 129 and 113 genes were up- and down-regulated, respectively. In addition, among the 2,375 proteins quantified, 39 and 23 proteins were up- and down-regulated, respectively. The gene expression patterns in diapause- and non-diapause were confirmed by qRT-PCR or western blot analysis. The overall analysis of robustly regulated genes at the protein and mRNA levels found four genes that overlapped in the up-regulated group and six genes in the down-regulated group, and thus these proteins/genes may regulate adult reproductive diapause. These differentially expressed proteins/genes act in the citrate cycle, insulin signaling pathway, PI3K-Akt signaling pathway, and amino acid biosynthesis pathways. These results provide the basis for further studies of the molecular regulation of reproductive diapause in this species.
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