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Enhancement of grain number per spike by RNA interference of cytokinin oxidase 2 gene in bread wheat

文献类型： 外文期刊

作者： Li, Yulian 1 ; Song, Guoqi 1 ; Gao, Jie 1 ; Zhang, Shujuan 1 ; Zhang, Rongzhi 1 ; Li, Wei 1 ; Chen, Mingli 1 ; Liu, Min 1 ;

作者机构： 1.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

2.Minist Agr, Natl Engn Lab Wheat & Maize, Key Lab Wheat Biol & Genet Improvement North Yell, Jinan 250100, Shandong, Peoples R China

3.Biogemma, Site Garenne,CS 90126, F-63720 Chappes, France

4.CAAS, Inst Crop Sci, Natl Wheat Improvement Ctr, 12 Zhongguancun South St, Beijing 100081, Peoples R China

关键词： CKX2.4; Grain number per spike; Transgenic plant; Triticum aestivum

期刊名称：HEREDITAS （ 影响因子：3.271； 五年影响因子：2.807 ）

ISSN： 1601-5223

年卷期： 2018 年 155 卷

页码：

收录情况： SCI

摘要： Background: This study aimed to validate the function of CKX gene on grain numbers in wheat. Methods: we constructed and transformed a RNA interference expression vector of TaCKX2.4 in bread wheat line NB1. Southern blotting analysis was used to select transgenic plants with single copy. The expression of TaCKX2.4 gene was estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, the relation between expression of TaCKX2.4 gene and grain numbers was validated. Results: Totally, 20 positive independent events were obtained. Homozygous lines from 5 events with a single copy of transformed gene each were selected to evaluate the expression of TaCKX2.4 and grain numbers per spike in T-3 generation. Compared with the control NB1, the average grain numbers per spike significantly increased by 12.6%, 8.3%, 6.5% and 5.8% in the T-3 lines JW39-3A, JW1-2B, JW1-1A and JW5-1A, respectively. Conclusion: Our study indicated that the expression level of TaCKX2.4 was negatively correlated with the grain number per spike, indicating that the reduced expression of TaCKX2.4 increased grain numbers per spike in wheat.

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