文献类型： 外文期刊
作者： Li, Hongwei 1 ; You, Yinwei 2 ; Zhang, Long 1 ;
作者机构： 1.China Agr Univ, Dept Entomol, Beijing, Peoples R China
2.Shandong Acad Agr Sci, Biotech Res Ctr, Jinan, Shandong, Peoples R China
关键词： Neuroscience; Issue 136; Single sensillum recordings; palp; olfaction; sensilla basiconica; chemosensory neuron; locust
期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.355； 五年影响因子：1.696 ）
ISSN： 1940-087X
年卷期： 2018 年 136 期
页码：
收录情况： SCI
摘要： The palps of locust mouthparts are considered to be conventional gustatory organs that play an important role in a locust's food selection, especially for the detection of non-volatile chemical cues through sensilla chaetica (previously named terminal sensilla or crested sensilla). There is now increasing evidence that these palps also have an olfactory function. An odorant receptor (LmigOR2) and an odorant-binding protein (LmigOBP1) have been localized in the neurons and accessory cells, respectively, in the sensilla basiconica of the palps. Single sensillum recording (SSR) is used for recording the responses of odorant receptor neurons, which is an effective method for screening active ligands on specific odorant receptors. SSR is used in functional studies of odorant receptors in palp sensilla. The structure of the sensilla basiconica located on the dome of the palps differs somewhat from the structure of those on the antennae. Therefore, when performing an SSR elicited by odorants, some specific advice may be helpful for obtaining optimum results. In this paper, a detailed and highly effective protocol for an SSR from insect palp sensilla basiconica is introduced.
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