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Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot

文献类型： 外文期刊

作者： Wang, Ronghua 1 ; Mei, Yi 3 ; Xu, Liang 1 ; Zhu, Xianwen 4 ; Wang, Yan 1 ; Guo, Jun 3 ; Liu, Liwang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Jinan 250100, Shandong, Peoples R China

3.Yancheng Acad Agr Sci, Yancheng 224002, Jiangsu, Peoples R China

4.North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA

关键词： Differential abundance protein species (DAPS); Heat stress; iTRAQ; Thermotolerance

期刊名称：PLANTA （ 影响因子：4.116； 五年影响因子：4.316 ）

ISSN： 0032-0935

年卷期： 2018 年 247 卷 5 期

页码：

收录情况： SCI

摘要： Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish. Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 A degrees C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.

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[1]Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot. Wang, Ronghua,Xu, Liang,Wang, Yan,Liu, Liwang,Wang, Ronghua,Mei, Yi,Guo, Jun,Zhu, Xianwen. 2018

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