Antiviral activity of portulaca oleracea L. extracts against porcine epidemic diarrhea virus by partial suppression on myd88/NF-Kb activation in vitro
文献类型： 外文期刊
作者： Liu, Yueyue 1 ; Zhao, Lu 1 ; Xie, Yunhui 1 ; Chen, Zhi 3 ; Yang, Shifa 1 ; Yin, Bin 1 ; Li, Guiming 1 ; Guo, Huijun 2 ; Lin, 1 ;
作者机构： 1.Shandong Acad Agr Sci, Poultry Breeding Engn Technol Ctr Shandong Prov, Inst Poultry Sci, Shandong Prov Key Lab Poultry Dis Diag & Immunol, Jinan 250023, Shandong, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China
3.Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Shandong, Peoples R China
关键词： Portulaca oleracea L; extracts; PEDV; In vitro
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.664 ）
ISSN： 0882-4010
年卷期： 2021 年 154 卷
页码：
收录情况： SCI
摘要： Porcine epidemic diarrhea virus (PEDV), especially variants, causes a highly contagious enteric disease which could give rise to huge economic losses in the swine industry worldwide. Portulaca oleracea L. has been reported to regulate intestine disease and involved in viral infections. However, the underlying mechanisms of Portulaca oleracea L. extracts against PEDV have not been fully elucidated. In this study, the antiviral effects and potential mechanisms of Portulaca oleracea L. extracts against PEDV were investigated in vitro. We first examined the inhibitory effects of different Portulaca oleracea L. extracts on the PEDV(JX-16 strain) in vitro and found that the water extract of Portulaca oleracea L.(PO)could significantly inhibit PEDV replication by 92.73% on VH cells and 63.07% on Vero cells. Furthermore, time-course analysis showed PO inhibited PEDV replication during the adsorption period of infectious cycle. Western blot and indirect immunofluorescence assay indicated that PO down-regulated the S protein expression in a dose-dependent manner. In addition, our results demonstrated the ability of PO to inhibit PEDV replication in VH cells by down-regulating the cytokine levels (TNF-alpha,IL-22 and IFN-alpha) and inhibiting the NF-KB signaling pathway activated by PEDV. Thus, Portulaca oleracea L extracts have potential utility in the preventive and therapeutic strategies for PEDV infection.
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