Tracing Key Molecular Regulators of Lipid Biosynthesis in Tuber Development of Cyperus esculentus Using Transcriptomics and Lipidomics Profiling
文献类型： 外文期刊
作者： Wang, Liyan 1 ; Jing, Meiling 1 ; Ahmad, Naveed 1 ; Wang, Yifei 1 ; Wang, Yijin 1 ; Li, Jia 1 ; Li, Xiaowei 1 ; Liu, Weic 1 ;
作者机构： 1.Jilin Agr Univ, Engn Res Ctr Bioreactor & Pharmaceut Dev, Coll Life Sci, Changchun 130118, Peoples R China
2.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Jinan 250100, Peoples R China
3.Hainan Univ, Coll Trop Crops, Haikou 570228, Hainan, Peoples R China
关键词： Cyperus esculentus; lipidomics; lipid synthesis; triacylglycerol; RNA-seq; Saccharomyces cerevisiae; CePAH1
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN：
年卷期： 2021 年 12 卷 10 期
页码：
收录情况： SCI
摘要： Cyperus esculentus is widely representing one of the important oil crops around the world, which provides valuable resources of edible tubers called tiger nut. The chemical composition and high ability to produce fats emphasize the role of tiger nut in promoting oil crop productivity. However, the underlying molecular mechanism of the production and accumulation of lipids in tiger nut development still remains unclear. Here, we conducted comprehensive transcriptomics and lipidomics analyses at different developmental stages of tuber in Cyperus esculentus. Lipidomic analyses confirmed that the accumulation of lipids including glycolipids, phospholipids, and glycerides were significantly enriched during tuber development from early to mature stage. The proportion of phosphatidylcholines (PC) declined during all stages and phosphatidyl ethanolamine (PE) was significantly declined in early and middle stages. These findings implied that PC is actively involved in triacylglycerol (TAG) biosynthesis during the tubers development, whereas PE may participate in TAG metabolism during early and middle stages. Comparative transcriptomics analyses indicated several genomic and metabolic pathways associated with lipid metabolism during tuber development in tiger nut. The Pearson correlation analysis showed that TAG synthesis in different developmental stages was attributed to 37 candidate transcripts including CePAH1. The up-regulation of diacylglycerol (DAG) and oil content in yeast, resulted from the inducible expression of exogenous CePAH1 confirmed the central role of this candidate gene in lipid metabolism. Our results demonstrated the foundation of an integrative metabolic model for understanding the molecular mechanism of tuber development in tiger nut, in which lipid biosynthesis plays a central role.
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