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The mutation in nicotinic acetylcholine receptor beta 1 subunit may confer resistance to imidacloprid in Aphis gossypii (Glover)

文献类型： 外文期刊

作者： Shi, Xu-Gen 1 ; Zhu, Yu-Kun 1 ; Xia, Xiao-Ming 1 ; Qiao, Kang 1 ; Wang, Hong-Yan 2 ; Wang, Kai-Yun 1 ;

作者机构： 1.Shandong Agr Univ, Dept Plant Protect, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Cotton Res Ctr, Jinan 250100, Shandong, Peoples R China

关键词： Aphis gossypii (Glover); imidacloprid; resistance; mutation; nicotinic acetylcholine receptor

期刊名称：JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT （ 影响因子：0.435； 五年影响因子：0.484 ）

ISSN： 1459-0255

年卷期： 2012 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Neonicotinoid insecticides, such as imidacloprid, are selective agonists on the insect nicotinic acetylcholine receptors - their molecular target site, which are used extensively to control a variety of different pest species. Just like other classes of insecticides, resistance to neonicotinoids is a significant threat, which has been identified in several pest species, including the cotton aphid. Aphis gossypii (Glover), a major cotton pest in many parts of Asia. A 66.49-fold imidacloprid-resistant Aphis gossyph strain was established in our work alter selection for 60 generations. Analysis of the cDNA sequence of the nicotinic acetylcholine receptor (nAChR) alpha 1, alpha 2, alpha 3, alpha 4-1, alpha 4-2, beta 1 subunits and the functional extracellular region (ranging from loop A to the 1st transmembrane domain) of the nicotinic acetylcholine receptor alpha 5 subunit from the resistant strain revealed a single point mutation in the loop D region of the nAChR beta 1 subunit causing an arginine to threonine substitution (R8IT). This mutation has been identified to be a key determinant of neonicotinoid binding to nAChRs and this amino acid change results in reduced sensitivity to neonicotinoids, which confers a vertebrate-like character to the insect nAChRs. This result indicated that in cotton aphids the single mutation (R8IT) might confer imidacloprid resistance.

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