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Diagnostic Kompetitive Allele-Specific PCR Markers of Wheat Broad-Spectrum Powdery Mildew Resistance Genes Pm21, PmV, and Pm12 Developed for High-Throughput Marker-Assisted Selection

文献类型： 外文期刊

作者： Zhang, Xu 1 ; Wang, Wenrui 1 ; Liu, Cheng 2 ; Zhu, Shanying 3 ; Gao, Huiming 4 ; Xu, Hongxing 5 ; Zhang, Lipei 6 ; Song, Jiancheng 1 ; Song, Wenyue 1 ; Liu, Kaichang 2 ; He, Huagang 3 ; Ma, Pengtao 1 ;

作者机构： 1.Yantai Univ, Coll Life Sci, Yantai 264005, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

3.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China

4.Hebei Univ Sci & Technol, Coll Food & Biol, Shijiazhuang 050018, Hebei, Peoples R China

5.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Henan, Peoples R China

6.Yantai Jien Biol Sci & Technol Ltd, Yantai 265100, Shandong, Peoples R China

关键词： diagnostic markers; high-throughput MAS; KASP; wheat powdery mildew

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2021 年 105 卷 10 期

页码：

收录情况： SCI

摘要： Wheat powdery mildew is a devastating disease that seriously threatens yield worldwide. Utilization of host resistance is considered an effective strategy to minimize powdery mildew damage. Pm21, PmV, and Pm12 confer broad-spectrum resistance to wheat powdery mildew in China, of which Pm21 and PmV are allelic genes derived from the 6VS chromosome of Dasypyrum villosum, and Pm12 is derived from the 6SS chromosome of Aegilops speltoides and most likely orthologous to the former two genes. To accurately and efficiently transfer and pyramid these genes using marker-assisted selection (MAS), distinctive single-nucleotide polymorphisms (SNPs) among the exon sequences of Pm21, PmV, and Pm12 and their homologous sequences in the common wheat genome were identified and then used for developing diagnostic Kompetitive Allele-Specific PCR (KASP) markers. The markers were validated in different genotypes including transgenic vectors, transgenic lines, translocation lines, resistance stocks with documented Pm genes, and in multiple susceptible cultivars without Pm genes. As a result, we initially developed a KASP marker that can simultaneously diagnose Pm21, Pm12, and PmV. Subsequently, we obtained a highly diagnostic KASP marker for each of the three genes that could distinguish among the three genes and also accurately distinguish them from other resistant stocks with documented Pm genes and from multiple susceptible genotypes. Compared with previously reported markers, the highly diagnostic KASP markers developed in this study have the advantages of low cost, easy assay, accuracy, and potentially high throughput for MAS.

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