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Effect of simvastatin on culturing of kidney cells from pigs in vitro

文献类型： 外文期刊

作者： Liu, Guifen 1 ; Lin, Haichao 1 ; Fu, Jinlian 1 ; Wang, Aiguo 1 ;

作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

2.Minist Agr, Key Lab Anim Genet & Breeding, Beijing 100193, Peoples R China

3.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

关键词： Simvastatin;Nitric oxide;Total cholesterol;Pig kidney cells

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Statins is an inhibitor in the cholesterol biosynthesis pathway. Simvastatin (SIM) has been found to have other clinical benefits besides those resulted from its actions of reducing plasma level of low density lipoprotein (LDL) cholesterol. Both mevastatin (MEV) and parvastatin (PAR) can increase release of nitric oxide (NO) which is a potent inhibitor of platelet aggregation and endothelial cell conglutination. In this study, we found different concentrations of SIM had different effects on 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) mRNA expression and NO and total cholesterol (TC) in normal cultural pig kidney cells. NO and TC were measured by using colorimetry in 550 nm and 546 nm, respectively. HMGR mRNA expression was tested by RT-PCR. Results showed that HMGR mRNA expression had a significant difference (P < 0.05) between different concentration of SIM treatment (0, 5, 10, or 25 omol/l). HMGR mRNA expression and TC content decreased gradually with the elevation of SIM concentration. The content of NO increased with the elevation of SIM concentration, but the difference was not notable. SIM affects the expression of HMGR-CoA, TC and NO in normal cells, but the specific mechanism need to be further research.

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