Effect of Nicotinamide on Proliferation, Differentiation, and Energy Metabolism in Bovine Preadipocytes
文献类型： 外文期刊
作者： Liu, Xiaomu 1 ; Fu, Jinlian 1 ; Song, Enliang 2 ; Zang, Kun 2 ; Wan, Fachun 2 ; Wu, Naike 2 ; Wang, Aiguo 1 ;
作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
3.Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China
关键词： cell growth;lipolytic activity
期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）
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收录情况： SCI
摘要： This study examined the effects of nicotinamide on proliferation, differentiation., and energy metabolism in a primary culture of bovine adipocytes. After treatment of cells with 100-500 mu M nicotinamide, cell growth was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and cellular lipid content was assessed by Oil Red 0 staining and a triglyceride (TG) assay. Several factors related to energy metabolism, namely adenosine triphosphatase (ATPase) activity, nitric oxide (NO) content, nitric oxide synthase (NOS) activity, the number of mitochondria and the relative expression of glyceraldehydes-3-phosphate dehydrogenase (GAPDH), peroxisome proliferator-activated receptor-gamma (PPAR gamma) and inducible NOS (iNOS), were also investigated. Results showed that nicotinamide induced both proliferation and differentiation in bovine preadipocytes. Nicotinamide decreased NO production by inhibiting NOS activity and iNOS mRNA expression, and controlled lipolytic activity by increasing ATPase activity and the number of mitochondria. The present study provides further evidence of the effects of nicotinamide on lipid and energy metabolism, and suggests that nicotinamide may play an important role in the development of bovine adipose tissue in vivo. This emphasizes the importance of investigating bovine adipose tissue to improve our understanding of dairy cow physiology.
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