文献类型： 外文期刊
作者： Wang, Dan 1 ; Liu, Yuan-Xia 1 ; Yu, Qian 1 ; Zhao, Shu-Ping 2 ; Zhao, Juan-Ying 2 ; Ru, Jing-Na 2 ; Cao, Xin-You 3 ; Fang 1 ;
作者机构： 1.Qingdao Agr Univ, Coll Agron, Qingdao 266109, Shandong, Peoples R China
2.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Biol & Genet Improvement Triticeae Crops, Minist Agr,Inst Crop Sci, Beijing 100081, Peoples R China
3.Shandong Acad Agr Sci, Natl Engn Lab Wheat & Maize, Key Lab Wheat Biol & Genet Improvement, Crop Res Inst, Jinan 250100, Shandong, Peoples R China
4.Yangtze Univ, Coll Agr, Coll Agron, Jingzhou 434025, Peoples R China
关键词： calcium-dependent protein kinase; abiotic stresses; responsive mechanism; soybean hairy root; Arabidopsis
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2019 年 20 卷 23 期
页码：
收录情况： SCI
摘要： Plants have a series of response mechanisms to adapt when they are subjected to external stress. Calcium-dependent protein kinases (CDPKs) in plants function against a variety of abiotic stresses. We screened 17 CDPKs from drought- and salt-induced soybean transcriptome sequences. The phylogenetic tree divided CDPKs of rice, Arabidopsis and soybean into five groups (I-V). Cis-acting element analysis showed that the 17 CDPKs contained some elements associated with drought and salt stresses. Quantitative real-time PCR (qRT-PCR) analysis indicated that the 17 CDPKs were responsive after different degrees of induction under drought and salt stresses. GmCDPK3 was selected as a further research target due to its high relative expression. The subcellular localization experiment showed that GmCDPK3 was located on the membrane of Arabidopsis mesophyll protoplasts. Overexpression of GmCDPK3 improved drought and salt resistance in Arabidopsis. In the soybean hairy roots experiment, the leaves of GmCDPK3 hairy roots with RNA interference (GmCDPK3-RNAi) soybean lines were more wilted than those of GmCDPK3 overexpression (GmCDPK3-OE) soybean lines after drought and salt stresses. The trypan blue staining experiment further confirmed that cell membrane damage of GmCDPK3-RNAi soybean leaves was more severe than in GmCDPK3-OE soybean lines. In addition, proline (Pro) and chlorophyll contents were increased and malondialdehyde (MDA) content was decreased in GmCDPK3-OE soybean lines. On the contrary, GmCDPK3-RNAi soybean lines had decreased Pro and chlorophyll content and increased MDA. The results indicate that GmCDPK3 is essential in resisting drought and salt stresses.
- 相关文献
作者其他论文 更多>>
-
TaMYB-CC5 gene specifically expressed in root improve tolerance of phosphorus deficiency and drought stress in wheat
作者：Kong, Ya-Nan;Yan, Xue-Chun;Liu, Yuan-Xia;Wang, Xin-Rui;Lan, Jin-Hao;Zheng, Lei;Kong, Ya-Nan;Wang, Xin-Rui;Zhang, Jin-Peng;Wang, Chao;Hou, Ze-Hao;Chen, Jun;Zhou, Yong-Bin;Chen, Ming;Ma, You-Zhi;Xu, Zhao-Shi;Qi, Xue-Li;Cao, Xin-You;Zhang, Shuang-Xi;Liu, Yong-Wei;Zheng, Jia-Cheng;Xu, Zhao-Shi
关键词：MYB-CC transcription factor; Phosphorus deficiency; Drought; Root; ZIFLs genes
-
Lipid transport protein ORP2A promotes glucose signaling by facilitating RGS1 degradation
作者：Yu, Qian;Sun, Jialu;Sun, Mengyao;Ge, Lei;Zou, Wenjiao;Liu, Kui;Chao, Yanru;Zhang, Qianqian;Wang, Xiaodong;Wang, Xiaofei;Ge, Lei;Zou, Wenjiao;Liu, Kui;Zhang, Qianqian
关键词：
-
Methylation Levels in the Promoter Region of FHIT and PIAS1 Genes Associated with Mastitis Resistance in Xinjiang Brown Cattle
作者：Zhong, Liwei;Ma, Shengchao;Wang, Dan;Zhang, Menghua;Zhang, Xiaoxue;Xu, Lei;Wu, Cuiling;Dong, Mingming;Gou, Murong;Huang, Xixia;Zhong, Liwei;Tian, Yuezhen;He, Junmin;Tian, Kechuan
关键词：Xinjiang brown cattle; mastitis resistance; GWAS; DNA methylation; candidate genes; FHIT and PIAS1 genes
-
Inhibitory effect and underlying mechanism of cinnamon and clove essential oils on Botryosphaeria dothidea and Colletotrichum gloeosporioides causing rots in postharvest bagging-free apple fruits
作者：Wang, Dan;Wang, Guiping;Wang, Jinzheng;Zhai, Hao;Xue, Xiaomin
关键词：bagging-free apple; post-harvest pathogens; essential oil; cell membrane; transcriptome
-
Cinnamon essential oil vapor alleviates the reduction of aroma-related volatiles in cold-stored "Feicheng" peach using HS-GC-IMS
作者：Wang, Dan;Zhang, Jing;Zhai, Hao;Chen, Wen-Yu;Jiang, Yang
关键词：aroma volatiles; flesh browning; chilling injury; cinnamon essential oil; HS-GC-IMS
-
Longitudinal genome-wide association studies of milk production traits in Holstein cattle using whole-genome sequence data imputed from medium-density chip data
作者：Teng, Jun;Wang, Dan;Zhao, Changheng;Zhang, Xinyi;Tang, Hui;Wang, Wenwen;Ning, Chao;Zhang, Qin;Chen, Zhi;Yang, Zhangping;Liu, Jianfeng;Sun, Dongxiao;Li, Jianbin;Mei, Cheng
关键词：imputation; whole-genome sequence data; longitudinal GWAS; milk production traits; Holstein cattle
-
GmTDN1 improves wheat yields by inducing dual tolerance to both drought and low-N stress
作者：Zhou, Yongbin;Liu, Jun;Tang, Wensi;Yan, Jiji;Yan, Huishu;Bai, Xinxuan;Ge, Linhao;Lyu, Mingjie;Chen, Jun;Xu, Zhaoshi;Chen, Ming;Ma, Youzhi;Guo, Jinkao;Wang, Yanxia;Ji, Hutai;Chu, Xiusheng;Xiao, Kai;Qi, Xueli;Hu, Lin;Li, Hui;Hu, Mengyun
关键词：DREB-like transcription factor; drought; nitrogen deficiency; transgenic wheat (Triticum aestivum L; ); field trial
