文献类型： 外文期刊

作者： Zhou, Yongbin ¹ ; Liu, Jun ¹ ; Guo, Jinkao ² ; Wang, Yanxia ² ; Ji, Hutai ³ ; Chu, Xiusheng ⁴ ; Xiao, Kai ⁵ ; Qi, Xueli ⁶ ; Hu, Lin ⁶ ; Li, Hui ⁷ ; Hu, Mengyun ⁷ ; Tang, Wensi ¹ ; Yan, Jiji ¹ ; Yan, Huishu ¹ ; Bai, Xinxuan ¹ ; Ge, Linhao ¹ ; Lyu, Mingjie ¹ ; Chen, Jun ¹ ; Xu, Zhaoshi ¹ ; Chen, Ming ¹ ; Ma, Youzhi ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Crop Sci ICS, Natl Key Facil Crop Gene Resources & Genet Improv, Minist Agr,Key Lab Biol & Genet Improvement Triti, Beijing, Peoples R China

2.Shijiazhuang Acad Agr & Forestry Sci, Res Ctr Wheat Engn Technol Hebei, Shijiazhuang, Hebei, Peoples R China

3.Shanxi Acad Agr Sci, Inst Wheat Res, Linfen, Shanxi, Peoples R China

4.Shandong Acad Agr Sci, Crop Res Inst, Jinan, Peoples R China

5.Agr Univ Hebei Prov, Coll Agron, Baoding, Peoples R China

6.Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou, Peoples R China

7.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Hebei Lab Crop Genet & Breeding, Shijiazhuang, Hebei, Peoples R China

关键词： DREB-like transcription factor; drought; nitrogen deficiency; transgenic wheat (Triticum aestivum L; ); field trial

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）

ISSN： 1467-7644

年卷期： 2022 年 20 卷 8 期

页码：

收录情况： SCI

摘要： Genetically enhancing drought tolerance and nutrient use efficacy enables sustainable and stable wheat production in drought-prone areas exposed to water shortages and low soil fertility, due to global warming and declining natural resources. In this study, wheat plants, exhibiting improved drought tolerance and N-use efficacy, were developed by introducing GmTDN1, a gene encoding a DREB-like transcription factor, into two modern winter wheat varieties, cv Shi4185 and Jimai22. Overexpressing GmTDN1 in wheat resulted in significantly improved drought and low-N tolerance under drought and N-deficient conditions in the greenhouse. Field trials conducted at three different locations over a period of 2-3 consecutive years showed that both Shi4185 and Jimai22 GmTDN1 transgenic lines were agronomically superior to wild-type plants, and produced significantly higher yields under both drought and N-deficient conditions. No yield penalties were observed in these transgenic lines under normal well irrigation conditions. Overexpressing GmTDN1 enhanced photosynthetic and osmotic adjustment capacity, antioxidant metabolism, and root mass of wheat plants, compared to those of wild-type plants, by orchestrating the expression of a set of drought stress-related genes as well as the nitrate transporter, NRT2.5. Furthermore, transgenic wheat with overexpressed NRT2.5 can improve drought tolerance and nitrogen (N) absorption, suggesting that improving N absorption in GmTDN1 transgenic wheat may contribute to drought tolerance. These findings may lead to the development of new methodologies with the capacity to simultaneously improve drought tolerance and N-use efficacy in cereal crops to ensure sustainable agriculture and global food security.