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Characterization of monoclonal antibodies specific to wheat glutenin subunits and their correlation with quality parameters

文献类型： 外文期刊

作者： Li, Qiaoyun 1 ; Ji, Kangmin 1 ; Zhang, Yanzhen 1 ; An, Xueli 1 ; He, Zhonghu 3 ; Xia, Xianchun 3 ; Yan, Yueming 1 ;

作者机构： 1.Capital Normal Univ, Key Lab Genet & Biotechnol, Coll Life Sci, Beijing 100048, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetable Res, Jinan 250100, Shandong, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Wheat Improvement Ctr, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

4.Chinese Acad Agr Sci, Int Maize & Wheat Improvement Ctr CIMMYT, China Off, Beijing 100081, Peoples R China

关键词： antibody characterization;crop quality parameter

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

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收录情况： SCI

摘要： Li, Q., Ji, K., Zhang, Y., An, X., He, Z., Xia, X. and Yan, Y. 2009. Characterization of monoclonal antibodies specific to wheat glutenin subunits and their correlation with quality parameters. Can. J. Plant Sci. 89: 11-19. Immunochemical methods are very useful in predicting the quality of wheat and differentiating alleles. In order to prepare appropriate monoclonal antibodies, HMW-GS 1Bx13 and 1By16 from spelt wheat were used as antigens to immunize BALB/C mice. Four monoclonal antibodies (mAbs) were obtained and designated 24231, 24245, 14588 and 14587, respectively. Results of Western blot showed that mAbs 24231 and 24245 prepared against 1Bx13 bound only to LMW-GS. The mAb 14588 prepared against 1By16 bound strongly to LMW-GS, but weakly to I By and some 1Dy type HMW-GS. The mAb 14587 prepared against 1By16 bound only to 1Dx HMW-GS. The results of indirect ELISA and statistical analysis showed that correlations between mAb 24231 and development time and stability were significantly (P<0.05) and highly significantly (P<0.01) negative, respectively, whereas those of mAb 24245 with development time and extensibility were highly significantly (P<0.01) and significantly (P<0.05) negative, respectively. Significantly (P<0.05) and highly significantly (P<0.01) positive correlations were observed between mAb 14588 and stability and development time. Mean differential binding of mAb 14587 with 1Dx5(t) and 1Dx2(t) subunits from Aegilops tauschii was highly significant (P<0.01), suggesting that ELISA could potentially be used as an effective screening tool during direct genetic transfer of desirable glutenin subunits from Aegilops tauschii to hexaploid wheat.

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