文献类型： 外文期刊

作者： Liu, Zh. J. ¹ ; Yang, X. H. ² ; Fu, Y. ² ;

作者机构： 1.Shandong Acad Agr Sci, Hitech Res Ctr, Jinan 250100, Peoples R China

2.China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China

关键词： high-oil maize;SAD;2-DE;gas chromatography;real-time PCR

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

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收录情况： SCI

摘要： As special maize with more than 6% oil concentration in the grain, high-oil maize has received increased interest recently. To date, little is known about the expressions of genes involved in fatty acid metabolism of high-oil maize. Stearoyl-acyl carrier protein desaturase (SAD) is a key enzyme that converts stearic acid to oleic acid. In this study, two-dimensional electrophoresis, gas chromatography, and real-time PCR were used to determine the expressions of SAD at three seed development stages in high-oil and normal maize inbred lines. SAD was significantly more abundantly expressed in high-oil maize than in normal maize, not only at the protein and mRNA levels, but also at the product level. These results suggested that a high expression of SAD may play an important role in increasing oil concentration in high-oil maize.