Efficient influence of ssDNA virus PCV2 replication by CRISPR/Cas9 targeting of the viral genome
文献类型： 外文期刊
作者： Shi, Jianli 1 ; Zheng, Shuxuan 4 ; Wu, Xiaoyan 1 ; Peng, Zhe 1 ; Li, Chen 1 ; Wang, Shuo 1 ; Xin, Changxun 1 ; Xu, Shaoji 1 ;
作者机构： 1.Shandong Acad Agr Sci, Div Swine Dis, Shandong Prov Key Lab Anim Dis Control & Breeding, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China
2.Qingdao Agr Univ, Qingdao 266000, Peoples R China
3.Shandong Normal Univ, Jinan 250014, Peoples R China
4.Univ Med Ctr Utrecht, Dept Med Microbiol, Utrecht, Netherlands
关键词： CRISPR/Cas9; Porcine circovirus type 2; Replication; sgRNA
期刊名称：MOLECULAR IMMUNOLOGY （ 影响因子：3.641； 五年影响因子：3.353 ）
ISSN： 0161-5890
年卷期： 2021 年 133 卷
页码：
收录情况： SCI
摘要： Porcine circovirus type 2 (PCV2), a ubiquitous pathogen that primary cause of postweaning multisystemic wasting syndrome (PMWS), had caused significant morbidity and mortality in swine populations with huge economic losses in the worldwide swine industry. Currently, looking for effective antiviral drugs for PCV2 infection remains an important works. In our study, CRISPR/Cas9 system was used to further detected the key sites of PCV2 replication. We designed 8 single guide RNAs (sgRNA) by targeting essential genes across the genome of PCV2. Western-blot(WB), Cell counting kit-8 for high-throughput sgRNA screening were applied to detect PCV2 replication levels. The results showed that Oc8, O13, O134, NQT and NPS sgRNAs can edit the PCV2 genome efficiently and inhibit PCV2 replication in PK-15 cell; H3 sgRNA cannot edit the PCV2 genome successfully; NAT sgRNA can edit the PCV2 genome efficiently to improve the PCV2 replication in PK-15 cell; O26 sgRNA can edit the PCV2 genome successfully but it is not known yet of its effect on PCV2 replication, besides the Cas9 expression had no effect on cell viability. These data suggest that CRISPR/Cas9 system targeting PCV2 essential genes may serve as a novel therapeutic agent against PCV2 infection in the future.
- 相关文献
作者其他论文 更多>>
-
Analysis of genetic variations of a recombinant strain derived from the three lineages of porcine reproductive and respiratory syndrome virus-2
作者：Zheng, Limei;Ma, Yingru;Wen, Jianxin;Li, Jun;Han, Xianjie;Wu, Xiaoyan;Liu, Chang;Li, Chen;Shi, Jianli;Han, Hong;Xu, Shaojian;Hrabchenko, Nataliia;Li, Jun;Huang, Mengjie;Qiu, Jianhua
关键词：PRRSV; recombination strains; GP5; NSP2; genetic evolutionary analysis; next-generation sequencing
-
Optimized production of full-length PCV2d virus-like particles in Escherichia coli : A cost-effective and high-yield approach for potential vaccine antigen development
作者：Zhang, Chengxin;He, Fang;Li, Nianfeng;Wen, Jianxin;Han, Xianjie;Li, Jun;Wu, Xiaoyan;Shi, Jianli;Li, Chen;Liu, Chang;Xu, Shaojian;Han, Hong;Hrabchenko, Nataliia;Du, Wei;Li, Jun
关键词：PCV2d; Cap protein; Virus -like particles (VLPs); Escherichia coli expression system; Vaccine development; Immunogenicity
-
Grp94 Inhibitor HCP1 Suppressed the Replication of SVA in BHK-21 Cells and PK-15 Cells
作者：Wang, Shuo;Cui, XiaoLing;Hui, Ren;Yao, Wen;Miao, JunYing;Zhao, BaoXiang;Li, Jun
关键词：Senecavirus A; Grp94; HCP1; Virus replication; Autophagy; Infection; Endoplasmic Reticulum (ER).
-
Development of SYBR Green I-based quantitative PCR assay for identification of porcine circovirus 1, 2 and 3
作者：Liu, Chang;Tian, Yao;Shi, Jian-Li;Li, Chen;Xu, Shao-Jian;Wu, Xiao-Yan;Han, Hong;Li, Jun;Tian, Yao;Zheng, Li-mei;Ma, Ying-Ru;Li, Jun;Han, Xian-Jie
关键词：porcine circovirus; specificity; sensitivity; SYBR Green I based quantitative PCR; single identification method
-
A novel VP2/VP3 recombinant of Senecavirus A isolated in northern China
作者：Ma, Yingru;Zheng, Limei;Li, Jun;Han, Xianjie;Li, Chen;Wu, Xiaoyan;Shi, Jianli;Liu, Chang;Han, Hong;Xu, Shaojian;Hrabchenko, Nataliia;Li, Jun;Huang, Mengjie;Qiu, Jianhua
关键词：senecavirus A; pigs; virus isolation; IFA; evolutionary analysis; Western blotting
-
METTL14 and FTO mediated m(6)A modification regulate PCV2 replication by affecting miR-30a-5p maturity
作者：Li, Chen;Wu, Xiaoyan;Shi, Jianli;Liu, Chang;Han, Hong;Xu, Shaojian;Li, Jun;Wu, Xiaoyan;Yang, Ying;Li, Jun;Wang, Shuo;Li, Jiaxin;Tian, Yao;Han, Xianjie;Ma, Yingru;Zheng, Limei;Li, Jun
关键词：Porcine circovirus type 2; m(6)A modification; miRNA maturity; replication; >
-
Effects of Eucommia ulmoides Oliver leaves on gut microbiota and intestinal function during in vitro simulated digestion and fermentation
作者：Wang, Xianshu;Fan, Shoujin;Zhang, Yu;Li, Chen;Fan, Shoujin;Liu, Wei;Zhang, Chao;Ji, Shuxia;Zhang, Yu;Wang, Xianshu
关键词：Eucommia ulmoides Oliver leaves; In vitro simulated digestion; In vitro fermentation; Gut microbiota; Caco-2/THP1 co-cultures; Inflammation
