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De novo and comparative transcriptome analysis of genetic male sterile and fertile lines in radish (Raphanus sativus)

文献类型： 外文期刊

作者： Duan, Naibin 1 ; Bai, Jing 1 ; Xie, Kun 1 ; Wang, Junfeng 1 ; Wang, Xiaomu 1 ;

作者机构： 1.Shandong Acad Agr Sci, Shandong Ctr Crop Germ Plasm Resources, Jinan, Shandong, Peoples R China

关键词： Radish breeding; genetic male sterility (GMS); differentially expressed genes; RNAseq; Denovo transcriptome analysis

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN： 1462-0316

年卷期： 2020 年 95 卷 1 期

页码：

收录情况： SCI

摘要： In radish, male sterility studies mainly focus on cytoplasmic male sterility (CMS) while genetic male sterility (GMS) has received less attention. To explore global gene expression profiles and identify genes associated with GMS, we performed de novo and comparative transcriptome analyses of flower buds from a GMS line and maintainer (fertile) line. Our study produced approximately 700 million paired-end reads, which were assembled into 115,284 unigenes with an average length of 1,196 bp and N50 of 1,729 bp. These unigenes were extensively annotated, with nearly 60% of them having homologs in the databases and 50% assigned with Gene Ontology (GO) terms. A total of 3,390 differentially expressed genes (DEGs) between the GMS line and the maintainer line were identified, with 2,645 down-regulated and 745 up-regulated in the GMS line. GO analyses showed biological processes related to plant reproductive development such as gametophyte development, pollen wall assembly, pollen development and pollen exine formation, as well as those related to cell wall development such as cell wall oganization and cell wall biogenesis, were significantly different in these two lines. The transcriptome assembly and comparative transcriptome analysis provides novel insights into the GMS in radishfacilitating the application of GMS in radish hybrid breeding.

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[1]De novo and comparative transcriptome analysis of genetic male sterile and fertile lines in radish (Raphanus sativus). Duan, Naibin,Bai, Jing,Xie, Kun,Wang, Junfeng,Wang, Xiaomu.

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