文献类型： 外文期刊

作者： Mao, Yunfei ¹ ; Cui, Xueli ¹ ; Wang, Haiyan ¹ ; Qin, Xin ¹ ; Liu, Yangbo ¹ ; Yin, Yijun ¹ ; Su, Xiafei ¹ ; Tang, Juan ² ; Wang, Fengling ² ; Ma, Fengwang ³ ; Duan, Naibin ⁴ ; Zhang, Donglin ⁵ ; Hu, Yanli ¹ ; Wang, Wenli ¹ ; Wei, Shaochong ¹ ; Chen, Xiaoliu ¹ ; Mao, Zhiquan ¹ ; Chen, Xuesen ¹ ; Shen, Xiang ¹ ;

作者机构： 1.Shandong Agr Univ, Coll Hort Sci & Engn, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China

2.Biomarker Technol Corp, Beijing, Peoples R China

3.Northwest Agr & Forestry Univ, Coll Hort, Yangling, Shaanxi, Peoples R China

4.Shandong Acad Agr Sci, Germplasm Resource Ctr Shandong Prov, Jinan, Peoples R China

5.Univ Georgia, Dept Hort, Athens, GA 30602 USA

关键词： Elaeagnus angustifolia L; Genome; Evolutionary response mechanism; Desertification-affected land

期刊名称：PLANT METHODS （ 影响因子：5.827； 五年影响因子：5.904 ）

ISSN：

年卷期： 2022 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Background Elaeagnus angustifolia L. is a deciduous tree in the family Elaeagnaceae. It is widely used to study abiotic stress tolerance in plants and to improve desertification-affected land because of its ability to withstand diverse types of environmental stress, such as drought, salt, cold, and wind. However, no studies have examined the mechanisms underlying the resistance of E. angustifolia to environmental stress and its adaptive evolution. Methods Here, we used PacBio, Hi-C, resequencing, and RNA-seq to construct the genome and transcriptome of E. angustifolia and explore its adaptive evolution. Results The reconstructed genome of E. angustifolia was 526.80 Mb, with a contig N50 of 12.60 Mb and estimated divergence time of 84.24 Mya. Gene family expansion and resequencing analyses showed that the evolution of E. angustifolia was closely related to environmental conditions. After exposure to salt stress, GO pathway analysis showed that new genes identified from the transcriptome were related to ATP-binding, metal ion binding, and nucleic acid binding. Conclusion The genome sequence of E. angustifolia could be used for comparative genomic analyses of Elaeagnaceae family members and could help elucidate the mechanisms underlying the response of E. angustifolia to drought, salt, cold, and wind stress. Generally, these results provide new insights that could be used to improve desertification-affected land.