您好，欢迎访问山东省农业科学院文献资源数据库平台

Significant Inhibition of Porcine Epidemic Diarrhea Virus In Vitro by Remdesivir, Its Parent Nucleoside and beta-d-N-4-hydroxycytidine

文献类型： 外文期刊

作者： Xie, Yuanchao 1 ; Guo, Xiaozhen 2 ; Hu, Tianwen 1 ; Wei, Daibao 1 ; Ma, Xiuli 2 ; Wu, Jiaqiang 2 ; Huang, Bing 2 ; Shen, 1 ;

作者机构： 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

2.Shandong Acad Agr Sci, Inst Poultry Sci, Jinan 250023, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词： Porcine epidemic diarrhea virus (PEDV); Nucleoside analog; RNA dependent RNA polymerase (RdRp); Antiviral activity

期刊名称：VIROLOGICA SINICA （ 影响因子：3.242； ）

ISSN： 1674-0769

年卷期：

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is widespread in the world. In recent years, the increased virulence of the virus due to viral variations, has caused great economic losses to the pig industry in many countries. It is always worthy to find effective therapeutic methods for PED. As an important class of antivirals, nucleoside drugs which target viral polymerases have been applied in treating human viral infections for half a century. Herein, we evaluated the anti-PEDV potential of three broad-spectrum antiviral nucleoside analogs, remdesivir (RDV), its parent nucleoside (RDV-N) and beta-d-N-4-hydroxycytidine (NHC). Among them, RDV-N was the most active agent in Vero E6 cells with EC50 of 0.31 mu mol/L, and more potent than RDV (EC50 = 0.74 mu mol/L) and NHC (EC50 = 1.17 mu mol/L). The activity of RDV-N was further confirmed using an indirect immuno-fluorescence assay. Moreover, RDV-N exhibited a good safety profile in cells and in mice. The high sequence similarity of the polymerase functional domains of PEDV with other five porcine coronaviruses indicated a broader antiviral spectrum for the three compounds. Generally, RDV-N is a promising broad-spectrum antiviral nucleoside, and it would be worthy to make some structural modifications to increase its oral bioavailability.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台