Engineering Trichoderma reesei for Hyperproduction of Cellulases on Glucose to Efficiently Saccharify Pretreated Corncobs
文献类型： 外文期刊
作者： Zheng, Fanglin 1 ; Yang, Renfei 1 ; Cao, Yanli 1 ; Zhang, Weixin 1 ; Lv, Xinxing 1 ; Meng, Xiangfeng 1 ; Zhong, Yaohua 1 ;
作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Inst Microbial Technol, Qingdao 266237, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc, Minist Agr & Rural Affairs,Inst Agrofood Sci & Te, Jinan 250100, Peoples R China
3.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
关键词： transcriptional activator; Xyr1; cellulase; Bgl1; Trichoderma reesei
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2020 年 68 卷 45 期
页码：
收录情况： SCI
摘要： The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is widely used as a cellulase producer in the industry. Herein, we describe the rational engineering of the publicly available T. reesei QM9414 strain to achieve a remarkable high-level production of cellulase on glucose. Overexpression of the key cellulase regulator XYR1 by the copper-repressible promoter Ptcu1 was first implemented to achieve a full cellulase production in the context of catabolite repression (CCR) while eliminating the requirement of inducing sugars for enzyme production. The T. reesei bgl1 gene was further overexpressed to compensate for its low beta-glucosidase activity on glucose. This overexpression resulted in a 102% increase in FPase activity compared with the CCR-released RUT-C30 strain cultured on Avicel. Moreover, the saccharification efficiency toward pretreated corncob residues by crude enzymes from the engineered strain on glucose increased by 85% compared with that treated by enzymes from RUT-C30 cultivated on Avicel. The engineered T. reesei strain thus shows great potential as a viable alternative to deliver commercial cellulases after further optimization for efficient saccharification of agricultural waste.
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