High-level production of pullulan from high concentration of glucose by mutagenesis and adaptive laboratory evolution of Aureobasidium pullulans
文献类型： 外文期刊
作者： Li, Xiwen 1 ; Zhao, Shuangzhi 2 ; Chen, Leilei 2 ; Zhou, Qingxin 2 ; Qiu, Jiying 2 ; Xin, Xue 2 ; Zhang, Yanhao 2 ; Yuan, Wei 2 ; Tian, Chengsen 4 ; Yang, Jinyu 2 ; Yu, Xiaobin 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Peoples R China
3.Key Lab Agroprod Proc Technol Shandong Prov, Jinan 250100, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Novel Food Resources Proc, Jinan 250100, Peoples R China
5.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
6.Qilu Normal Univ, Sch Chem & Chem Engn, Jinan 250200, Peoples R China
关键词： Pullulan; Aureobasidium pullulans; Adaptive laboratory evolution; High glucose concentration
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：11.2； 五年影响因子：10.2 ）
ISSN： 0144-8617
年卷期： 2023 年 302 卷
页码：
收录情况： SCI
摘要： The cost of carbon sources and the low efficiency of the fermentation titer limit the industrial application of pullulan. In this study, a hypertonic-tolerant strain with efficient utilization of glucose was obtained using a double strategy. Initially, a strain for efficient synthesis of pullulan from glucose was generated by mutagenesis. Subsequently, the mutant was directionally evolved on the plate containing a high glucose concentration to enhance high osmotic resistance. The enzyme activities and the transcriptional levels involved in pullulan biosynthesis and high osmotic tolerance in mutants were increased. Nitrogen source and inorganic salts also significantly affected the production of pullulan by M233-20 from high concentration of glucose. The pullulan titer of 162.1 g/L was obtained using the response surface methodology in the flask. The strain M233-20 pro-duced 162.3 g/L pullulan in a 30-L bioreactor with a yield of 0.82 g/g glucose. Hence, this work provides a potential industrial pullulan producer M233-20 and a strategy to develop excellent strain.
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