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Transcriptome analysis reveals the crucial function of hyperoside in inhibiting anthocyanin accumulation in grape (Vitis vinifera L.) fruits by inducing VvMYB62

文献类型： 外文期刊

作者： Su, Ling 1 ; Zhang, Man 2 ; Zhang, Yudie 2 ; Chen, Yingchun 1 ; Yang, Liying 1 ; Wang, Yongmei 1 ; Song, Yangbo 4 ; Gong, Lei 1 ;

作者机构： 1.Shandong Acad Grape, Shandong Acad Agr Sci, Jinan, Peoples R China

2.Hebei Normal Univ Sci & Technol, Coll Hort Sci & Technol, Wuhan, Hebei, Peoples R China

3.Hebei Key Lab Hort Germplasm Excavat & Innovat Uti, Qinhuangdao, Hebei, Peoples R China

4.Qinghai Univ, Coll Agr & Anim Husb, Xining, Peoples R China

关键词： anthocyanin; grape; hyperoside; transcriptome; veraison

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： IntroductionThe formation of color in plants is significantly dependent on anthocyaninpigments. Grape species vary in color due to the differences in anthocyanin accumulation. It is widely recognized that both biotic and abiotic conditions may have an impact on anthocyanin synthesis in plants. The underlying molecular mechanisms by which external application of hyperoside impacts anthocyanin formation in grapes, however, have received little attention. MethodsIn the current study,the transcriptome of Gemstone seedless grape was examined using high-throughput RNA sequencing at various developmental stages reply to both control and hyperoside treatments. ResultsThe results of this study suggested that the major genes controlling anthocyanin accumulation in response to the externalinjection of hyperoside could be VvMYB62, VvPAL, VvCHS, and VvF3'5'H.Quantitative reverse transcription PCR (RT-qPCR) results were used to confirm the changes in the expression levels of the genes encoding the anthocyanin biosynthesis pathway under the control and hyperoside treatments. Using a transient transformation system, it was discovered that VvMYB62 was shown to regulate the anthocyanin accumulation at both the transcriptional and posttranslational levels and could be influenced by the external administration of hyperoside. In grape embryogenic calli, hyperoside could specifically suppress theexpression of VvMYB62 and anthocyanin accumulation. In this instance, the VvMYB62 characterisation brought attention to the significance of exogenous hyperoside-induced anthocyanin accumulation. Therefore, the results demonstrated that VvMYB62 could be hindered in the process of grape during anthocyanin accumulation caused by hyperoside. DiscussionThese findings offer excellent candidate genes in the future breeding of novel grape varieties in addition to serving as a crucial reference for understanding the underlying molecular processes of hyperoside suppression of anthocyanin formation in plants.

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