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A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus

文献类型： 外文期刊

作者： Zhang, Lianzhi 1 ; Zhao, Qiaoya 2 ; Tian, Ye 2 ; Tang, Yi 1 ; Wang, Yixin 1 ; Huang, Bing 2 ;

作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Peoples R China

2.Inst Poultry Sci, Shandong Acad Agr Sci, Jinan, Peoples R China

关键词： rabbit hemorrhagic disease virus; GI; 1 genotype; isothermal; recombinase polymerase amplification; rapid detection method; epidemiological surveillance

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： Rabbit Viral Hemorrhagic Disease (RHD) is a highly contagious and fatal infection, resulting in considerable economic losses to the rabbit industry. Consequently, it is essential to develop a fast and accurate diagnostic method for RHDV GI.1. In this study, a rapid simple reverse transcriptase recombinase polymerase amplification (RTRPA) for RHDV GI.1 was successfully developed using specific primers to RHDV GI.1 VP60 gene. Results indicated that the entire amplification process could be achieved in an isothermal condition at 40 degrees C for 30 minutes, with good specificity and no reaction to other common rabbit disease pathogens, and a high sensitivity of upto 0.1LD(50) of RHDV GI.1. Then, RT-RPA method was used to detect 1144 clinical samples, and the positive rates were 0.95%, 1.29% and 2.50% in Zaozhuang, Linyi, and Liaocheng in Shandong Province, respectively (the Fisher's exact test, P = 0.413), suggesting that there is no significant difference in RHDV GI.1 infection among the different regions. In conclusion, this study established a RT-RPA assay which is suitable for quick detection and monitoring of RHDV GI.1, thus making it a viable option for epidemiological surveillance.

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