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De novo full length transcriptome analysis and gene expression profiling to identify genes involved in phenylethanol glycosides biosynthesis in Cistanche tubulosa

文献类型： 外文期刊

作者： Hou, Lei 1 ; Li, Guanghui 1 ; Chen, Qingliang 2 ; Zhao, JinJin 3 ; Pan, Jiaowen 1 ; Lin, Ruxia 1 ; Zhu, Xiujin 1 ; Wang, Pengfei 4 ; Wang, Xingjun 1 ;

作者机构： 1.Shandong Acad Agr Sci, Shandong Prov Key Lab Crop Genet Improvement Ecol, Inst Crop Germplasm Resources, Jinan 250100, Peoples R China

2.Beijing Univ Chinese Med, Modern Res Ctr Tradit Chinese Med, Beijing 100029, Peoples R China

3.Shandong Acad Grape, Shandong Engn Res Ctr Grape Cultivat & Deep Proc, Jinan 250100, Peoples R China

4.Hebei Agr Univ, Coll Agron, Baoding 071000, Peoples R China

关键词： Cistanche tubulosa; Full-length transcriptome; PacBio; RNA-seq; Phenylethanol glycosides

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background The dried stem of Cistanche, is a famous Chinese traditional medicine. The main active pharmacodynamic components are phenylethanol glycosides (PhGs). Cistanche tubulosa produces higher level of PhGs in its stems than that of Cistanche deserticola. However, the key genes in the PhGs biosynthesis pathway is not clear in C. tubulosa. Results In this study, we performed the full-length transcriptome sequencing and gene expression profiling of C. tubulosa using PacBio combined with BGISEQ-500 RNA-seq technology. Totally, 237,772 unique transcripts were obtained, ranging from 199 bp to 31,857 bp. Among the unique transcripts, 188,135 (79.12%) transcripts were annotated. Interestingly, 1080 transcripts were annotated as 22 enzymes related to PhGs biosynthesis. We measured the content of echinacoside, acteoside and total PhGs at two development stages, and found that the content of PhGs was 46.74% of dry matter in young fleshy stem (YS1) and then decreased to 31.22% at the harvest stage (HS2). To compare with YS1, 13,631 genes were up-regulated, and 15,521 genes were down regulated in HS2. Many differentially expressed genes (DEGs) were identified to be involved in phenylpropanoid biosynthesis pathway, phenylalanine metabolism pathway, and tyrosine metabolism pathway. Conclusions This is the first report of transcriptome study of C. tubulosa which provided the foundation for understanding of PhGs biosynthesis. Based on these results, we proposed a potential model for PhGs biosynthesis in C. tubulosa.

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