PREPARATIVE SEPARATION AND PURIFICATION OF SESQUITERPENOIDS FROM Tussilago farfara L. BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
文献类型： 外文期刊
作者： Wang, Daijie 1 ; Fang, Lei 1 ; Wang, Xiao 1 ; Qiu, Jiying 2 ; Huang, Luqi 3 ;
作者机构： 1.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Shandong, Peoples R China
3.Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 100700, Peoples R China
关键词： high-speed counter-current chromatography; sesquiterpenoids; Tussilago farfara L.
期刊名称：QUIMICA NOVA （ 影响因子：0.961； 五年影响因子：1.091 ）
ISSN： 0100-4042
年卷期： 2011 年 34 卷 5 期
页码：
收录情况： SCI
摘要： Preparative high-speed counter-current chromatography (HSCCC) was successfully applied for separation and purification of sesquiterpenoids from an extract of Tussilago farfara L. with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.5:1.1:0.3, v/v/v/v). The separation produced a total of 32 mg of tussilagone, 18 mg of 14-acetoxy-7 beta-(3'-ethyl cis-crotonoyloxy)-1 alpha-(2'-methyl butyryloxy)-notonipetranone and 21 mg of 7 beta-(3'-ethyl cis-crotonoyloxy)-1 alpha-(2'-methyl butyryloxy)-3,14-dehydro-Z-notonipetranone from 500 mg of the crude extract in one step separation with the purity of 99.5, 99.4 and 99.1%, respectively, as determined by HPLC. The structures of these compounds were identified by ESI-MS, (1)H-NMR and (13)C-NMR.
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