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Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage

文献类型： 外文期刊

作者： Li, Qiaoyun 1 ; Tong, Haishen 4 ; Zhang, Zhigang 1 ; Zhao, Zhizhong 1 ; Song, Xiyun 4 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Vegetable Res, Jinan 250100, Peoples R China

2.Natl Improvement Ctr Vegetable, ShanDong Branch, Jinan 250100, Peoples R China

3.Key Lab Biol Greenhouse Vegetable Shandong Prov, Jinan 250100, Peoples R China

4.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China

关键词： Chinese cabbage; turnip mosaic virus; genetic analysis; expressed sequence tags-simple sequence repeat markers

期刊名称：CANADIAN JOURNAL OF PLANT SCIENCE （ 影响因子：1.018； 五年影响因子：1.242 ）

ISSN： 0008-4220

年卷期： 2011 年 91 卷 4 期

页码：

收录情况： SCI

摘要： Li, Q., Tong, H., Zhang, Z., Zhao, Z. and Song, X. 2011. Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Can. J. Plant Sei. 91: 707-715. Turnip mosaic virus (TuMV) is one of the major pathogens infecting Brassica crops (including Chinese cabbage), and often causes serious reductions in yield and quality. Breeding for resistant cultivars is complicated by the existence of numerous TuMV strains and isolates. The objective of this research was to determine the mode of inheritance and to develop molecular markers associated with TuMV resistance in Chinese cabbage. F(1) and F(2) populations were developed from the cross between 71-36-2 (susceptible) and 73 (resistant) lines and mechanically inoculated with TuMV-C4. Inheritance analysis by visual scoring and enzyme-linked immunosorbent assay (ELISA) indicated that resistance to TuMV-C4 in this cross was controlled by one recessive gene. A total of 132 EST-SSR primers were designed from EST sequences available in public databases. Seven primers detected polymorphism between parental genotypes. Marker HCC259 was associated with the TuMV resistance Ph-retr02. The distance between the marker and the TuMV resistance gene retr02 was 3.8 cM. This is the first co-dominant marker linked to the TuMV resistance gene with a distance less than 5.0 cM in Chinese cabbage. This marker was suitable for TuMV-C4 resistance screening in progenies from the cross between lines 73 (resistant) and susceptible line, 71-36-2. The usefulness of this marker was validated in 21 additional resistant and susceptible lines. This marker has the potential to simplify and accelerate breeding Chinese cabbage cultivars resistant to TuMV-C4.

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