文献类型： 外文期刊

作者： Zhang, HQ ¹ ; Liu, YQ ² ; Liu, B ² ; Gao, PJ ² ;

作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China; Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

关键词： numerical differential method;instantaneous growth rate;growth parameters;bacterial population

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2006 年 49 卷 2 期

页码：

收录情况： SCI

摘要： Using mathematical analysis, a new method has been developed for studying the growth kinetics of bacterial populations in batch culture. First, sampling data were smoothed with the spline interpolation method. Second, the instantaneous rates were derived by numerical differential techniques and finally, the derived data were fitted with the Gaussian function to obtain growth parameters. We named this the Spline-Numerical-Gaussian or SNG method. This method yielded more accurate estimates of the growth rates of bacterial populations and new parameters. It was possible to divide the growth curve into four different but continuous phases based on changes in the instantaneous rates. The four phases are the accelerating growth phase, the constant growth phase, the decelerating growth phase and the declining phase. Total DNA content was measured by flow cytometry and varied depending on the growth phase. The SNG system provides a very powerful tool for describing the kinetics of bacterial population growth. The SNG method avoids the unrealistic assumptions generally used in the traditional growth equations.