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Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes

文献类型： 外文期刊

作者： Yu, Shanlin 1 ; Pan, Lijuan 1 ; Yang, Qingli 2 ; Min, Ping 2 ; Ren, Zengkai 2 ; Zhang, Hongsheng 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Shandong Acad Agr Sci, Peanut Res Inst, Qingdao 266100, Peoples R China

关键词： peanut;Delta(12) fatty acid desaturase;oleic acid;yeast expression;function identification

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2008 年 35 卷 11 期

页码：

收录情况： SCI

摘要： Delta(12) fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trail in peanut seeds. By RT-PCR method, the full-length cDNAs of Delta(12) fatty acid desaturase gene were isolated from peanut (A rachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2B', respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAWB' sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2B', with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Delta(12) fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2B' proved that only AhFAD2B gene product showed significant Delta(12) fatty acid desaturase, activity, but AhFAD2B' gene product did not. These results suggested that the change of AhFAD2B' gene sequence resulted in lower activity or deactivation of Delta(12) fatty acid desaturase in high oleic acid genotype.

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