Genetic variation and pathogenicity of highly virulent porcine reproductive and respiratory syndrome virus emerging in China
文献类型： 外文期刊
作者： Wu, J. 1 ; Li, J. 1 ; Tian, F. 2 ; Ren, S. 1 ; Yu, M. 1 ; Chen, J. 2 ; Lan, Z. 2 ; Zhang, X. 1 ; Yoo, Dongwan 3 ; Wang, Jinbao 1 ;
作者机构： 1.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Shandong, Peoples R China
2.Shandong Ctr Anim Dis Control & Prevent, Jinan, Shandong, Peoples R China
3.Univ Illinois, Dept Pathobiol, Coll Vet Med, Urbana, IL 61802 USA
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2009 年 154 卷 10 期
页码：
收录情况： SCI
摘要： A highly pathogenic swine disease designated as 'porcine high fever disease (PHFD)' appeared recently in China. Porcine reproductive and respiratory syndrome virus (PRRSV) was identified as an agent associated with PHFD, and two discontiguous sequence deletions were identified as a genetic marker in the Nsp2 region of the viral genome. To examine PHFD in Shandong province, a total of 10 PRRSV isolates were recovered from pig herds that had never been vaccinated for PRRS. Sequence analysis of open reading frame 5 (ORF5) showed that the level of identity among the 10 isolates ranged between 88.2 and 99.2%. For the non-structural protein 2 (Nsp2) gene, three isolates shared high sequence identity with VR-2332, the prototype virus of the North American genotype, while the remaining seven isolates exhibited two discontiguous sequence deletions that were identical to those of PHFD: a one-amino-acid (phenylalanine) deletion at position 482 and a 29-amino-acid deletion at positions 533-561 of Nsp2. Experimental infection of pigs with SD-JN, which was one of the seven isolates containing such deletions, resulted in severe clinical symptoms characterized by red discoloration on the body and hemorrhages in the lungs, kidneys, and inguinal lymph nodes, accompanied by higher mortality and longer duration of viremia. These symptoms were similar to those of PHFD observed in the field. Our results show that VR2332-like PRRSV coexists with PHFD-associated atypical PRRSV in pig herds in the Shandong area, and different PRRSV isolates differ greatly in their pathogenesis and virulence in pigs.
- 相关文献
作者其他论文 更多>>
-
GTPase activity of porcine Mx1 plays a dominant role in inhibiting the N-Nsp9 interaction and thus inhibiting PRRSV replication
作者：Hu, Yue;Wu, Xiangju;Tian, Yunfei;Jiang, Dandan;Ren, Jinrui;Li, Ziyong;Zhang, Quanfang;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Ding, Xiuliang;Yoo, Dongwan;Miller, Laura C.;Lee, Changhee;Lee, Changhee
关键词：porcine reproductive and respiratory syndrome virus; interferons; antiviral agents; Mx1
-
Reprogramming viral immune evasion for a rational design of next-generation vaccines for RNA viruses
作者：Su, Chia-Ming;Rowland, Raymond R. R.;Yoo, Dongwan;Du, Yijun;Wang, Qiuhong;Wang, Qiuhong
关键词：type I interferons (IFNs); NF-kappa B (NF-kappa B); IFN antagonism; live-attenuated vaccine; veterinary vaccine; viral immune evasion; next-generation vaccines; veterinary virology
-
Immobilized lignin peroxidase on chitosan-modified halloysite nanotubes for degradation of polycyclic aromatic hydrocarbons in soil
作者：Chai, C.;Ji, Y.;Wang, N.;Ge, W.;Wu, J.;Wang, Y. Q.;Li, Y.
关键词：Lignin peroxidase; Halloysite nanotubes; Immobilization; PAHs; Degradation
-
Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication
作者：Wu, Xiangju;Hu, Yue;Sui, Chao;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Wu, Xiangju;Hu, Yue;Sui, Chao;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Pan, Li;Yoo, Dongwan;Miller, Laura C.;Lee, Changhee;Lee, Changhee
关键词：3C protease; foot-and-mouth disease virus (FMDV); SUMOylation; replication
-
Inhibition of Antiviral Innate Immunity by Foot-and-Mouth Disease Virus L-pro through Interaction with the N-Terminal Domain of Swine RNase L
作者：Sui, Chao;Jiang, Dandan;Wu, Xiangju;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Sui, Chao;Liu, Sidang;Li, Feng;Li, Feng;Pan, Li;Yoo, Dongwan;Rock, Daniel L.;Miller, Laura C.;Lee, Changhee
关键词：FMDV; RNase L; L-pro; antagonistic mechanism; ISGs; foot-and-mouth disease virus
-
beta-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle
作者：Wei, C.;Tan, X.;Liu, G.;Wan, F.;Zhao, H.;You, W.;Liu, X.;Zhang, X.;Jin, Q.;Zhang, C.
关键词：adipose tissue; beef cattle; beta-carotene; gene expression; lipid metabolism
-
Physiological and molecular responses of two Chinese cabbage genotypes to heat stress
作者：Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Cui, B.;Qiu, N.;Yang, F.;Qiu, N.
关键词：antioxidants; chlorophyll fluorescence; heat shock; malondialdehyde; proline; proteins; sugars; transcription factor
